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Prostaglandin D₂ (PGD₂) acts through two G protein-coupled receptors (GPCRs), the prostanoid DP receptor and CRTH2 also known as DP1 and DP2, respectively. Several previously characterized GPCR antagonists are now classified as inverse agonists and a number of GPCR ligands are known to display pharmacochaperone activity towards a given receptor. Here, we demonstrate that a DP1 specific antagoni...
(3R)-4-(4-Chlorobenzyl)-7-fluoro-5-(methylsulfonyl)-1,2,3,4-tetrahydrocyclopenta[b]indol-3-yl acetic acid (MK-0524) is a potent orally active human prostaglandin D(2) receptor 1 antagonist that is currently under development for the prevention of niacin-induced flushing. The major in vitro and in vivo metabolite of MK-0524 is the acyl glucuronic acid conjugate of the parent compound, M2. To com...
Sorrel (Rumex acetosa L.) is a model dioecious plant in genetic and molecular studies for sex determination. In this research, gus reporter gene transformation to leaf disks of this plant via Agrobacterium tumefaciens was evaluated based on transient expression of this gene. Three strains of Agrobacterium( LBA4404, C58 and EHA101) and two kinds of bacterial suspensions (suspension I: Agrobacter...
[(3R)-4-(4-Chlorobenzyl)-7-fluoro-5-(methylsulfonyl)-1,2,3,4tetrahydrocyclopentaindol-3-yl]acetic acid (MK-0524) is a potent orally active human prostaglandin D2 receptor 1 antagonist that is currently under development for the prevention of niacin-induced flushing. The metabolism and excretion of [C]MK-0524 in humans were investigated in six healthy human volunteers following a single p.o. dos...
Sorrel (Rumex acetosa L.) is a model dioecious plant in genetic and molecular studies for sex determination. In this research, gus reporter gene transformation to leaf disks of this plant via Agrobacterium tumefaciens was evaluated based on transient expression of this gene. Three strains of Agrobacterium( LBA4404, C58 and EHA101) and two kinds of bacterial suspensions (suspension I: Agrobacter...
Currently, domestic production of vegetable soybean (aka “edamame”) lags well behind consumer demand, with approximately 70% U.S.-consumed edamame imported each year. A major barrier for growth the U.S. industry is an overall lack varieties adequate acceptability and adaption to climate environment. In this study, we evaluated eleven genotypes (including one commercial check) differences in yie...
Phytate represents an organic pool of phosphorus in soil that requires hydrolysis by phytase enzymes produced microorganisms prior to its bioavailability plants. We tested the ability a microbial suspension made from old growth maple forest’s undisturbed mineralize phytate greenhouse trial on soybean plants inoculated or non-inoculated with suspension. MiSeq Amplicon sequencing targeting bacter...
objective: traditional methods for storing microbial dna samples use the low-temperatures of 4 °c, -20°c or -80 °c. the dbc card is a new method of storing microbial dna at room temperature. this study evaluates the stability and determine the best storing method of bacterial dna on the dbc card. methods: in this study, we used a pair of primers from the conserved domain of 16srrna designed to...
[(3R)-4-(4-Chlorobenzyl)-7-fluoro-5-(methylsulfonyl)-1,2,3,4-tetrahydrocyclopentaindol-3-yl]acetic acid (MK-0524) is a potent orally active human prostaglandin D(2) receptor 1 antagonist that is currently under development for the prevention of niacin-induced flushing. The metabolism and excretion of [(14)C]MK-0524 in humans were investigated in six healthy human volunteers following a single p...
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