1Member of JSCE, Associate Professor, Crisis & Security Management Research Center, Kagawa University (2217-20 Hayashi-cyo, Takamatsu, 761-0396, Japan) E-mail: [email protected] 2Member of JSCE, Graduate School of Kagawa University (2217-20 Hayashi-cyo, Takamatsu, 761-0396, Japan) E-mail: [email protected] 3Member of JSCE, Professor, Crisis & Security Management Researc...

Mibefradil is a Ca2+ channel antagonist that inhibits both T-type and high-voltage-activated Ca2+ channels. We previously showed that block of high-voltage-activated channels by mibefradil occurs through the production of an active metabolite by intracellular hydrolysis. In the present study, we modified the structure of mibefradil to develop a nonhydrolyzable analog, (1S, 2S)-2-(2-(N-[(3-benzi...

A novel mibefradil derivative, NNC55-0396, designed to be hydrolysis-resistant, was shown to be a selective T-type Ca(2+) channel inhibitor without L-type Ca(2+) channel efficacy. However, its effects on cytochromes P450 (P450s) have not previously been examined. We investigated the inhibitory effects of NNC55-0396 toward seven major recombinant human P450s--CYP3A4, CYP2D6, CYP1A2, CYP2C9, CYP2...

Accumulating evidence reports that T-type calcium channels play crucial roles in tumor formation and development. However, the of inhibiting cells with various inhibitors progression remain unclear. This study aimed to investigate cytotoxic apoptotic effects NNC 55-0396, a channel inhibitor, against SNU-1 gastric cancer cells. Cytotoxic 55-0396 were evaluated by XTT assay flow cytometry. The re...

BACKGROUND T-type Ca(2+) channels are often aberrantly expressed in different human cancers and participate in the regulation of cell cycle progression, proliferation and death. METHODS RT-PCR, Q-PCR, western blotting and whole-cell patch-clamp recording were employed to assess the expression of T-type Ca(2+) channels in leukemia cell lines. The function of T-type Ca(2+) channels in leukemia ...

There is concern regarding neurotoxicity induced by the use of local anesthetics. A previous study showed that an overload of intracellular calcium is involved in the neurotoxic effect of some anesthetics. T-type calcium channels, which lower the threshold of action potentials, can regulate the influx of calcium ions. We hypothesized that T-type calcium channels are involved in bupivacaine-indu...

http://dx.doi.org/10.1016/j.engstruct.2014.10.028 0141-0296/ 2014 Elsevier Ltd. All rights reserved. ⇑ Corresponding author at: EPFL ENAC IIC EESD, GC B2 484 (Bâtiment GC), Station 18, 1015 Lausanne, Switzerland. E-mail addresses: [email protected] (J.P. Almeida), [email protected] (A.A. Correia), [email protected] (R. Pinho). 1 Address: LNEC-DE/NESDE, Av. do Brasil 101, 1700-066 Lisbon, Po...

1 Pediatric, Pulmonary, and Allergy Division, University of Florida, Gainesville, FL 32610-0296, USA 2Children’s Health and Exercise Research Centre, School of Sport and Health Sciences, University of Exeter, Exeter EX4 4QJ, UK 3Department Exercise Epidemiology, Institute of Sport Sciences and Clinical Biomechanics, University of Southern Denmark, DK-5270 Odense, Denmark 4Pediatrics, Allergy an...

Stem Durability Testing of Interdental Brush Masato HOTTA1, Shoichi IMADE2, Akira SANO2, Hiroshi NAKAJIMA3 and Kohji YAMAMOTO1 1Asahi University School of Dentistry , Division of Oral Functional Science and Rehabilitation, Department of Prosthodontics, 1851 Hozumi, Mizuho-city, Gifu 501-0296 Japan 2Jacks Co . Ltd., 3-3 Choeiji, Higashiosaka-city, Osaka 577-0055, Japan 3Meikai University , Schoo...

Voltage-gated Ca(2+) channels (Ca(V)) are ubiquitously expressed in various cell types and play vital roles in regulation of cellular functions including proliferation. However, the molecular identities and function of Ca(V) remained unexplored in preadipocytes. Therefore, whole cell voltage-clamp technique, conventional/quantitative real-time RT-PCR, Western blot, small interfering RNA (siRNA)...