background and objective: time-temperature indicators are used in smart packaging, and described as intelligent tools attached to the label of food products to monitor their timetemperature history. since the previous studies on microbial time-temperature indicators were only based on ph-dependent changes, and they were long-time response indicators, in the present work, a new microbial time-te...

alpha-amylase is widely used as an industrial enzyme, and a therapeutic target for which inhibitors are designed. organic solvents are used to dissolve various compounds that would be studied as moderators of alpha-amylases, but they could themselves affect enzyme activity and stability. methanol, ethanol and propanol are simple alcohols that may be commonly used to this end, and their effect h...

Thermostable α-amylase is of great importance in the starch fermentation industry; it is extensively used in the manufacture of beverages, baby foods, medicines, and pharmaceuticals. Bacillus amyloliquefaciens produces thermostable α-amylase; however, production of thermostable α-amylase is limited. Ion-beam implantation is an effective method for mutation breeding in microbes. We conduct...

Bacillus amyloliquefaciens a-amylase (1,4-a-D-glucan glucanohydrolase. EC 3.2.1.1), which is commercially supplied as 'Bacillus subtilis a-amylase' does not cross-react immunologically with B. subtilis a-amylase. This enzyme (from B. amyloliquefaciens) was cleaved by treatment with CNBr into seven fragments. Peptide A was selected for sequence determination. It is the longest one, containing 18...

Eco-enzymes are one of the potential sources for obtaining amylolytic bacterial isolates. The study aims to screen bacteria from eco-enzymes, characterize semi-purification amylase, and identify molecularly using 16S rRNA gene. Screening was carried out by measuring index on a Nutrient agar medium containing 1% tapioca starch. isolate which had highest non-pathogenic selected amylase characteri...

the thermal denaturation of α-amylase from bacillus amyloliquefaciens has been investigated in the presence and absence of sodium dodecyl sulphate (sds) over the temperature range (293-373) k in 20 mm sodium phosphate buffer, ph 6.9, using temperature scanning spectroscopy. the presence of sds caused the destabilization of α-amylase resulting in a decrease in the temperature of unfolding with a...

Bacillus amyloliquefaciens strain JJC33M is a bacterium that produces α-amylase (EC 3.2.1.1) and was isolated from sugarcane soil. Its estimated genome size is 3.96 Mb, and it harbors 4,048 coding genes (CDSs).

The phylogenetic relationships of 17 Bacillus strains isolated from plants and soil were determined from partial sequences of genes encoding 16S rRNA, gyraseA (gyrA) and the cheA histidine kinase. Five strains were closely related to Bacillus subtilis subsp. subtilis, three strains were more closely related to B. subtilis subsp. spizizeni and two strains were identified as B. mojavensis. The re...

Alpha amylase family is generally defined as a group of enzymes that can hydrolyse and transglycosylase α-(1, 4) or α-(1, 6) glycosidic bonds along with the preservation of anomeric configuration. For the comparative analysis of alpha amylase family, nucleotide sequences of seven thermo stable organisms of Kingdom Archea i.e. Pyrococcus furiosus (100-105°C), Kingdom Prokaryotes i.e. Bacillus li...

Thermostable α-amylases hold a very important place in commercial industrial applications Sri Lanka. Therefore, the main aim of this study was to identify superior Bacillus strain and optimize growth conditions that could yield high α-amylase production. Three strains, B. amyloliquefaciens ATCC 23350, licheniformis 14580 megaterium 14581 were used for study. Shake flask culture experiments cond...