The highly specific restriction endonucleases Sa1I and BamI produce DNA fragments with complementary, cohesive termini that can be covalently joined by DNA ligase. The Escherichia coli kanamycin resistance factor pML21 has one SalI site, at which DNA can be inserted without interfering with the expression of drug resistance or replication of the plasmid. A more convenient cloning vehicle can be...

abstract salinity is one of the major abiotic challenges influencing plant productivity worldwide. to examine the response of two alfalfa cultivars (bami and hamedani) to six levels of water salinity (0, 25, 50, 75, 100, 125 mm l-1 nacl), a glasshouse experiment was conducted at the college of agriculture, shiraz university, shiraz, iran in 2008. the results showed that in bami (which appeared...

Abstract Salinity is one of the major abiotic challenges influencing plant productivity worldwide. To examine the response of two alfalfa cultivars (Bami and Hamedani) to six levels of water salinity (0, 25, 50, 75, 100, 125 mM L-1 NaCl), a glasshouse experiment was conducted at the College of Agriculture, Shiraz University, Shiraz, Iran in 2008. The results showed that in Bami (which appeared...

salt stress is one of the most chalanging abiotic stresses affecting natural productivity and causing significant croplosses worldwide. a greenhouse experiment was conducted to evaluate the response of two alfalfa cultivars (bamiand hamedani) to 6 levels of salinity (0 as control, 25, 50, 75, 100, 125 mm nacl) at the college of agriculture,shiraz university, shiraz, iran in 2008. plant dry weig...

Salt stress is one of the most chalanging abiotic stresses affecting natural productivity and causing significant croplosses worldwide. A greenhouse experiment was conducted to evaluate the response of two alfalfa cultivars (Bamiand Hamedani) to 6 levels of salinity (0 as control, 25, 50, 75, 100, 125 mM NaCl) at the College of Agriculture,Shiraz University, Shiraz, Iran in 2008. Plant dry weig...

In this work, we compared the profile of proteins secreted by planktonic and biofilm cultures of Pseudomonas aeruginosa using two-dimensional difference gel electrophoresis (2D-DiGE). This revealed that a novel metzincin protease, Mep72, was secreted during biofilm growth. Subsequent Western blotting and reverse transcription-PCR (RT-PCR) analyses demonstrated that Mep72 was expressed only duri...

In 2006, the Task Force of the European Society of Cardiology published its consensus document on the use of autologous cell therapy for repair of the heart. Since then, there have been numerous clinical trials and analyses performed to establish the role of autologous cell therapy in the treatment of both acute and chronic cardiac disease. The majority of these studies have been Phase II clini...