abstract background and aims: newcastle disease (nd) is a highly contagious disease that affects many species of birds and causes significant economic losses to the poultry industry worldwide and the pathogenicity of newcastle disease virus (ndv) strains varies with different virulence. we aim to detect lentogenic (low virulence) nd virus (nd/ir 2010) using sentinel birds and molecular detectio...

infectious bronchitis (ib) and newcastle disease (nd) are highly contagious and the most economically important diseases of the poultry affecting respiratory tract and causing economic losses in poultry industry throughout the world. in the present study, the simultaneous detection and differentiation of causative agents of these diseases were investigated using duplex-rt-pcr. rna was extracted...

in order to adapt lasota strain of newcastle disease virus (ndv) on chick embryo fibroblast (cef) cells, 0.1 ml from lasota vaccine produced in razi vaccine and serum research institute inoculated to the cef cells and was passaged five times in the cef grown in eagle’s minimum essential medium (mem). first to third passages were blind but in fourth passage cytopathic effect of virus was observe...

newcastle disease (nd) is one of the most important diseases that affect birds; the epizootic nature of the disease has caused severe economic losses in the poultry industry worldwide. in this experiment nd virus (ndv) was inactivated by two different chemicals binary ethylenimine (bei) and formaldehyde. formaldehyde was used at 0.1%, while bei was used at concentrations of 1 to 4 mm. ndv inact...

Rural poultry farming is common in the Northern provinces. Similar to commercial poultry, rural poultry is susceptible to most infectious diseases. In addition, by increasing the density of poultry farming, the probability of disease incidences has been increased. Newcastle disease is the most highly infectious disease which is endemic in Iran and causes outbreaks among commercial and rural pou...

newcastle disease virus (ndv) was isolated and identified for the first time from a flock of japanese quail suspected to nd in iran. birds of the flock aged variously, and some of them died from the disease. loss of appetite, weakness and decrease in egg production, diarrhea and nervous symptoms were the main clinical signs in the birds. hemorrhagic lesions of the intestinal tracts and proventr...

background: the specific antibody response against newcastle disease virus (ndv) in the blood serum of chickens hatched from eggs exposed to low dose gamma-radiation was studied. materials and methods: two groups of eggs of commercial meat chicken lines were irradiated with the dose of 0.30 gy 60co gamma-rays before incubation and on the 19th day of incubation, respectively. the same number of ...

newcastle disease (nd) is one of the most serious illnesses of chickens. live vaccines are widely used to prevent chicken from the disease all over the world. to access the effective and potentiate nd vaccine, a homogenous subpopulation from lasota strain was selected following cultivation of the virus on primary chicken embryofibroblast (cef) cells. pathogenicity indices and molecular characte...

objectives the current research aimed to study achillea millefolium and thymus vulgaris antiviral activity against newcastle disease virus (ndv). materials and methods the antiviral activity of the plants was measured by the reduction assay of viral titer, and explained by inhibition percentage (ip). background nowadays natural products such as pure compounds and plant extract scan provide unli...

background and aims: ndv (newcastle disease virus) is one of the viruses that cause disease in avian with severe economic losses in the poultry industry in many countries. fusion protein (f) which plays a major role in the virus pathogenicity contains several regions that have a role in the fusion process. mutation in the sequence of hr1 & hr2 regions of this protein prevents fusion of the viru...