نتایج جستجو برای: ژن aroA

تعداد نتایج: 16027  

ژورنال: :مجله تحقیقات دامپزشکی (journal of veterinary research) 2009
بهار نیری فسایی تقی زهرایی صالحی حسن تاجبخش

ژن aroa یکی از ژن های مهم برای تولید اسید آمینه های آروماتیک می باشد و برای حیات باکتری بسیار ضروری است. نشان داده شده است، که توالی ژنی aroa در باکتری ها متفاوت است، بنابر این هدف این مطالعه تعیین توالی نوکلئوتیدی ژن aroa در سویه اشریشیا کلی بومی 80: k78o برای مشخص کردن تفاوت ها بین این سویه و سویه های موجود در بانک ژنی اشریشیا کلی 80: k78o بود. برای ازدیاد ژن aroa در سویه بومی از واکنش زنجیره...

ژورنال: تحقیقات دامپزشکی 2009

‌ژن‌aroA یکی از ژن‌های مهم برای تولید اسید آمینه‌های آروماتیک می‌باشد و برای حیات باکتری‌ بسیار ضروری است. نشان داده شده است، که توالی ژنی aroA در باکتری‌ها متفاوت است، بنابر این هدف این مطالعه تعیین توالی نوکلئوتیدی ژن aroA در سویه‌ ‌اشریشیا کلی‌ ‌بومی‌ 80: K78O برای‌ ‌مشخص کردن تفاوت‌ها بین این سویه و سویه‌های موجود در بانک ژنی اشریشیا کلی ‌80: K78O بود. برای ازدیاد ژن aroA در سویه بومی از و...

Journal: :Applied and environmental microbiology 2005
Yi-Cheng Sun Yan-Cheng Chen Zhe-Xian Tian Feng-Mei Li Xin-Yue Wang Jing Zhang Zhen-Long Xiao Min Lin Niamh Gilmartin David N Dowling Yi-Ping Wang

Glyphosate has been used globally as a safe herbicide for weed control. It inhibits 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase (AroA), which is a key enzyme in the aromatic amino acid biosynthetic pathway in microorganisms and plants. A Pseudomonas putida strain, 4G-1, was isolated from a soil heavily contaminated by glyphosate in China. Its AroA-encoding gene (aroA) has been cloned, se...

2012
Gaoyi Cao Yunjun Liu Shengxue Zhang Xuewen Yang Rongrong Chen Yuwen Zhang Wei Lu Yan Liu Jianhua Wang Min Lin Guoying Wang

A key enzyme in the shikimate pathway, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) is the primary target of the broad-spectrum herbicide glyphosate. Identification of new aroA genes coding for EPSPS with a high level of glyphosate tolerance is essential for the development of glyphosate-tolerant crops. In the present study, the glyphosate tolerance of five bacterial aroA genes was evalu...

Journal: :Journal of bacteriology 2001
G Gosset C A Bonner R A Jensen

Enzymes performing the initial reaction of aromatic amino acid biosynthesis, 2-keto-3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthases, exist as two distinct homology classes. The three classic Escherichia coli paralogs are AroA(I) proteins, but many members of the Bacteria possess the AroA(II) class of enzyme, sometimes in combination with AroA(I) proteins. AroA(II) DAHP synthases un...

Journal: :Journal of general microbiology 1993
C O'Connell P A Pattee T J Foster

The aroA gene of Staphylococcus aureus 8325-4 was cloned. Sequence analysis and the phenotype of directed plasmid insertions 5' to aroA suggest that aroA is located in an operon and that it maps 3' to the aroC and aroB genes. A revised consensus sequence for the aroA gene product EPSP synthase binding site for its substrate (phosphoenolpyruvate) and an inhibitor (glyphosate) is proposed. An aro...

Journal: :Infection and immunity 1990
M Roberts D Maskell P Novotny G Dougan

A DNA fragment encoding a kanamycin resistance determinant was used to insertionally inactivate the cloned aroA gene of Bordetella pertussis in Escherichia coli K-12, and a conjugative shuttle vector system based on the suicide vector pRTP1 was used to deliver the mutations from E. coli back into B. pertussis CN2992FS and BP1. The aroA mutation was introduced by allelic exchange into the chromo...

Journal: :Infection and immunity 2002
Mohammad Tabatabaei Zhiqi Liu Anna Finucane Roger Parton John Coote

Hemorrhagic septicemia (HS) is a fatal systemic disease of cattle and buffaloes. In South Asia HS is caused by infection with Pasteurella multocida serotype B:2. Some control is achieved with alum-precipitated or oil-adjuvanted killed whole-cell vaccines injected subcutaneously, but these vaccines provide only short-term immunity and require annual administration for effective use. Live attenua...

Journal: :Journal of microbiology and biotechnology 2014
Xiao-Juan Xing Yong-Sheng Tian Ri-He Peng Jing Xu Wei Zhao Quan-Hong Yao Sheng Sun

Although a large number of AroA enzymes (EPSPS: 5-enopyruvylshikimate-3-phosphate synthase) have been identified, cloned, and tested for glyphosate resistance, only two AroA variants, derived from Agrobacterium tumefaciens strain CP4 and Zea mays, have been utilized to produce the commercial glyphosate-resistant crops. Here, we have used a PCR-based twostep DNA synthesis method to synthesize an...

A. R. Jabbari, G. R. Moazzeni Jula M. Esmailzadeh M. Tabatabaei,

Haemorrhagic septicaemia (HS) is a fatal systemic disease of cattle and buffaloes. Some control isachieved with administration of alum-precipitated or oil-adjuvanted killed whole-cell vaccines injectedsubcutaneously. These vaccines, however, provide only short-term immunity and for effective use, theyshould be administered annually. We constructed an aroA attenuated derivative of a Pasteurella ...

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