Downlo GRP3 mediates the activation of the Ras signaling pathway that is present in many human cancers. Here, lored the involvement of RasGRP3 in the formation and maintenance of the prostate cancer phenotype. P3 expression was elevated inmultiple human prostate tumor tissue samples and in the human androgenndent prostate cancer cell lines PC-3 and DU 145 compared with the androgen-dependent pr...

ownload GRP3 mediates the activation of the Ras signaling pathway that is present in many human cancers. Here, lored the involvement of RasGRP3 in the formation and maintenance of the prostate cancer phenotype. P3 expression was elevated inmultiple human prostate tumor tissue samples and in the human androgenndent prostate cancer cell lines PC-3 and DU 145 compared with the androgen-dependent p...

We identified Ras guanine-releasing protein 3 (RasGRP3) as a guanine exchange factor expressed in blood vessels via an embryonic stem (ES) cell-based gene trap screen to identify novel vascular genes. RasGRP3 is expressed in embryonic blood vessels, down-regulated in mature adult vessels, and reexpressed in newly formed vessels during pregnancy and tumorigenesis. This expression pattern is cons...

Glioblastoma (GBM), the most aggressive primary brain tumors, are highly infiltrative. Although GBM express high Ras activity and Ras proteins have been implicated in gliomagenesis, Ras-activating mutations are not frequent in these tumors. RasGRP3, an important signaling protein responsive to diacylglycerol (DAG), increases Ras activation. Here, we examined the expression and functions of RasG...

nloaded GRP3 mediates the activation of the Ras signaling pathway that is present in many human cancers. Here, lored the involvement of RasGRP3 in the formation and maintenance of the prostate cancer phenotype. P3 expression was elevated inmultiple human prostate tumor tissue samples and in the human androgenndent prostate cancer cell lines PC-3 and DU 145 compared with the androgen-dependent p...

RATIONALE Fetuses that develop in diabetic mothers have a higher incidence of birth defects that include cardiovascular defects, but the signaling pathways that mediate these developmental effects are poorly understood. It is reasonable to hypothesize that diabetic maternal effects are mediated by 1 or more pathways activated downstream of aberrant glucose metabolism, because poorly controlled ...

The RasGRPs are a family of Ras activators that possess diacylglycerol-binding C1 domains. In T cells, RasGRP1 links TCR signaling to Ras. B cells coexpress RasGRP1 and RasGRP3. Using Rasgrp1 and Rasgrp3 single and double null mutant mice, we analyzed the role of these proteins in signaling to Ras and Erk in B cells. RasGRP1 and RasGRP3 both contribute to BCR-induced Ras activation, although Ra...

Members of the RasGRP family of Ras activators have C1 domains that bind diacylglycerol (DAG) and DAG analogs such as the tumor-promoting phorbol esters. RasGRP members could be responsible for some of the DAG signaling processes that have previously been attributed to protein kinase C (PKC). We found that RasGRP3 is selectively expressed in B cells, suggesting that RasGRP3 might function downs...

Two important Ras guanine nucleotide exchange factors, Son of sevenless (Sos) and Ras guanine nucleotide releasing protein (RasGRP), have been implicated in controlling Ras activation when cell surface receptors are stimulated. To address the specificity or redundancy of these exchange factors, we have generated Sos1/Sos2 double- or RasGRP3-deficient B cell lines and determined their ability to...