هدف: از چالش­های عمده بشریت افزایش افسردگی و اختلال عملکرد جنسی ناشی داروهای ضدافسردگی است. با توجه به نقش سلول­های سرتولی در اسپرماتوژنز، پژوهش حاضر، اثر داروی دولوکستین را بر زنده­مانی، آپوپتوزیس بیان ژن­های Bax و­ (Connexin 43) Cx43 بررسی کرده مواد روش‌‌ها: TM4 محیط DMEM/F12 حاوی %5/2 FBS، 5% سرم اسب %1 پنی سیلین-استرپتومایسین کشت شدند. دوزهای 30،60، 15، 5/7، 75/3 میکرو­گرم/ میلی­لیتر زمان­ه...

Activation of the tumor necrosis factor R1/Fas receptor results in the cleavage of cytosolic BID to truncated tBID. tBID translocates to the mitochondria to induce the oligomerization of BAX or BAK, resulting in the release of cytochrome c (Cyt c). Here we demonstrate that in tumor necrosis factor alpha-activated FL5.12 cells, tBID becomes part of a 45-kDa cross-linkable mitochondrial complex t...

The molecular basis of the interaction between mitochondrial carrier homologue 2 (MTCH2) and truncated BID (tBID) was characterized. These proteins participate in the apoptotic pathway, and the interaction between them may serve as a target for anticancer lead compounds. In response to apoptotic signals, MTCH2 recruits tBID to the mitochondria, where it activates apoptosis. A combination of pep...

In normal circumstances, the Bcl-2 family dutifully governs when cells die. However, the rules of engagement between the pro- and antiapoptotic family members are still contested, and how Bax is transformed from a cytosolic monomer to an outer mitochondrial membrane-permeabilizing oligomer is unclear. With fluorescence techniques and an in vitro system, the combination of tBid and Bax produced ...

Truncated BID (tBID), a proapoptotic BCL2 family protein, induces BAK/BAX-dependent release of cytochrome c and other mitochondrial intermembrane proteins to the cytosol to induce apoptosis. The voltage-dependent anion channels (VDACs) are the primary gates for solutes across the outer mitochondrial membrane (OMM); however, their role in apoptotic OMM permeabilization remains controversial. Her...