نتایج جستجو برای: ژنهای ctxa
تعداد نتایج: 1274 فیلتر نتایج به سال:
زمینه و اهداف: سویه های سم زای(toxogenic) ویبریوکلرا که واجد ژن های ctxa،ctxb و zot هستند، نسبت به سویه های غیرسم زابیماری شدیدتری ایجاد می کنند، بنابراین شناسائی دقیق و سریع سویه های سم زا بسیار حائز اهمیت است. این مطالعه با هدف به کارگیری روش multiplex pcr برای جداسازی هم زمان ژن های ctxa ، ctxb و zot در ویبریو کلرا ایزوله شده از بیماران طراحی گردید. مواد و روش کار: در این مطالعه 73 سویه بالی...
Bone mineral density (BMD) estimates for the proximal femur using Dual Energy X-ray Absorptiometry (DXA) are currently considered the standard for making a diagnosis of osteoporosis in an individual patient using BMD alone. We have compared BMD results from a commercial Quantitative CT (QCT) BMD analysis system, "CTXA Hip", which provides clinical data for the proximal femur, to results from DX...
The temporal expression patterns of the critical Vibrio cholerae virulence genes, tcpA and ctxA, were determined during infection using a recombinase reporter. TcpA was induced biphasically in two temporally and spatially separable events in the small intestine, whereas ctxA was induced monophasically only after, and remarkably, dependent upon, tcpA expression; however, this dependence was not ...
The Ibis T5000 is a novel diagnostic platform that couples PCR and mass spectrometry. In this study, we developed an assay that can identify all known pathogenic Vibrio species and field-tested it using natural water samples from both freshwater lakes and the Georgian coastal zone of the Black Sea. Of the 278 total water samples screened, 9 different Vibrio species were detected, 114 (41%) samp...
Cholera toxin (CT), a virulence factor elaborated by Vibrio cholerae, is sufficient to induce the severe diarrhea characteristic of cholera. The enzymatic moiety of CT (CtxA) increases cAMP synthesis in intestinal epithelial cells, leading to chloride ion (Cl(-)) efflux through the CFTR Cl(-) channel. To preserve electroneutrality and osmotic balance, sodium ions and water also flow into the in...
زمینه و اهداف: سویه‌های سم‌زای(Toxogenic) ویبریوکلرا که واجد ژنهای ctxA،ctxB و zot هستند، نسبت به سویه‌های غیرسم‌زابیماری شدیدتری ایجاد می‌کنند، بنابراین شناسائی دقیق و سریع سویه‌های سم‌زا بسیار حائز اهمیت است. این مطالعه با هدف به‌کارگیری روش Multiplex PCR برای جداسازی همزمان ژنهای ctxA ، ctxB و zot در ویبریو کلرا ایزوله‌شده از بیماران طراحی گردید. مواد و روش کار: در این مطالعه 73 سویه بالی...
Virulence-associated genes of Vibrio cholerae including O1, O139 and non-O1/non-O139 from an outbreak in Songkhla Province and sporadic cases occurred in Thailand during 1993 - 2002 were investigated. One hundred eighty-five V. cholerae strains were examined for the presence of virulence-associated genes including ctxA, tcpA, zot, toxR, toxS, toxT, and ace by polymerase chain reaction. DNA sequ...
The structural genes which constitute the cholera toxin operon, ctxAB, were genetically mapped in the Vibrio cholerae El Tor strain RV79. This strain of V. cholerae contains two copies of the ctx operon located on a 7-kilobase-pair tandemly duplicated region. We began by isolating a vibriophage VcA1 insertion mutation in one of the two ctxA genes located in this region. The mutant carrying this...
This study aimed at characterizing the phenotypic and toxigenic status of circulating strains of cholera during outbreaks in Nigeria, employing molecular typing techniques. Two hundred and one samples of rectal swabs, stool, vomitus, water (from the well, borehole, sachet, stream, and tap) and disinfectants (sodium hypochlorite) were collected from three states in the country. The samples were ...
Seawater and plankton samples were collected over a period of 17 months from November 1998 to March 2000 along the coast of Peru. Total DNA was extracted from water and from plankton grouped by size into two fractions (64 micro m to 202 micro m and >202 micro m). All samples were assayed for Vibrio cholerae, V. cholerae O1, V. cholerae O139, and ctxA by PCR. Of 50 samples collected and tested, ...
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