The distribution of napin and cruciferin, the two major storage proteins in rape seed, Brassica napus, has been visualized during seed development by antibody staining of paraffin-embedded and sectioned seeds. The results indicate that the synthesis of both proteins during embryogenesis is strictly regulated with respect to time and tissue. Although the synthesis of napin started a few days ear...

Rapeseed protein consists mainly of the seed storage cruciferin and napin. Cruciferin napin have different nutritional values techno-functional properties. Shifting cruciferin/napin ratio towards either more or could allow diversified applications. The objective this study was to investigate genetic variation in modern winter rapeseed cultivars. contents were analysed by SDS-PAGE. Genetic for b...

Transcription rates and mRNA levels for Brassica napus seed storage protein families, cruciferin and napin, have been determined in embryos developing in the seed, as well as in embryos cultured with and without abscisic acid. Cruciferin and napin mRNAs are high during the cell expansion phase of embryo development, representing as much as 11 and 8%, respectively, of the total embryo mRNA. Duri...

We have begun the molecular characterization of genes encoding napin, the 1.7 S embryo-specific storage protein of Brassica napus. Genomic Southern blot analysis indicates that napin is encoded by a multigene family comprised of a minimum of 16 genes. Two DNA fragments containing single napin genes have been recovered from B. napus genomic libraries. We have determined the nucleotide sequence o...

The two major storage proteins identified in Brassica napus (canola) were isolated and studied for their molecular composition, structural characteristics and the responses of structural features to the changes in pH and temperature. Cruciferin, a complex of six monomers, has a predominantly β-sheet-containing secondary structure. This protein showed low pH unstable tertiary structure, and dist...

The amino acid sequences of a number of closely related proteins ("napin") isolated from Brassica napus were determined by mass spectrometry without prior separation into individual components. Some of these proteins correspond to those previously deduced (napA, BngNAP1, and gNa), chiefly from DNA sequences. Others were found to differ to a varying extent (BngNAP1', BngNAP1A, BngNAP1B, BngNAP1C...

Proteins derived from plant resources such as oilseed meals, canola and sunflower, are considered a viable alternative to animal proteins for food consumption. This work presents rational methodology, using high throughput experimentation (HTE), the separation of cruciferin napin, two major meal, by chromatography. Eight different mixed mode ion exchange resins were evaluated at conditions with...

Storage protein gene expression, characteristic of mid- to late embryogenesis, was investigated in microspore embryos of rapeseed (Brassica napus). These embryos, derived from the immature male gametophyte, accumulate little or no detectable napin or cruciferin mRNA when cultured on hormone-free medium containing 13% sucrose. The addition of abscisic acid (ABA) to the medium results in an incre...