نتایج جستجو برای: مارکر srap
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در این مطالعه برای بررسی تنوع ژنتیکی بین 39 کلن صنوبر ((populus l. متعلق به چهار گونه p.deltoides, p. nigra, p.alba و p.euramericana از نشانگرهای srap و issr استفاده شد. نمونه های گیاهی از کلکسیون پایه ی مادری صنوبر در مرکز تحقیقات البرز وابسته به موسسه ی تحقیقات جنگل ها و مراتع جمع آوری شدند. در مجموع از 11 ترکیب پرایمری نشانگر srap، 42 باند و از 7 پرایمر issr، 81 باند تکثیر شد. به طور متوسط ه...
در تحقیق حاضر مارکر های مولکولی srap و issr جهت بررسی تنوع ژنتیکی در میان 15 نمونه از 13 گونه از جنس silene استفاده شدند. نمونه ها از نقاط مختلف ارومیه و خراسان جمع آوری شدند. 19 پرایمر srap، 62 باند قابل شمارش تولید کردند که 46 باند پلی مورف بودند(90/71 درصد). 13 پرایمر issr نیز 211 باند قابل شمارش ایجاد کردند که از میان آن ها 209 باند پلی مورف بودند(19/99 درصد). تشابه ژنتیکی از 47/0 تا 90/0 ب...
A segregating F4 population from the cross between drought sensitive (Yecora Rojo) and drought tolerant (Pavon 76) genotypes was made to identify SRAP markers linked to leaf chlorophyll content, flag leaf senescence and cell membrane stability traits in wheat (Triticum aestivum L.) under water-stressed condition. The parents and 150 F4 families were evaluated phenotypically for drought toleranc...
BACKGROUND Roles of Estrogen Receptor-beta 1 (ER-β1) and its co-regulator Steroid Receptor RNA Activator Protein (SRAP) in breast cancer remain unclear. Previously, ER-β1 and SRAP expression were found positively correlated in breast cancer and, therefore, expression of these two molecules could characterize cancers with a distinct clinical outcome. PATIENTS AND METHODS ER-β1 and SRAP express...
In a widely accepted model, the steroid receptor RNA activator protein (SRA protein; SRAP) modulates the transcriptional regulatory activity of SRA RNA by binding a specific stem-loop of SRA. We first confirmed that SRAP is present in the nucleus as well as the cytoplasm of MCF-7 breast cancer cells, where it is expressed at the level of about 10(5) molecules per cell. However, our SRAP-RNA bin...
Chinese kale is an original Chinese vegetable of the Cruciferae family. To select suitable parents for hybrid breeding, we thoroughly analyzed the genetic diversity of Chinese kale. Random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) molecular markers were used to evaluate the genetic diversity across 21 Chinese kale accessions from AVRDC and Guangzhou in ...
Sequence-related amplified polymorphism (SRAP) and inter-simple sequence repeat (ISSR) markers were used to assess the genetic diversity within and among 15 natural populations of Stipa bungeana from the Loess Plateau of China. Using 15 SRAP primers, 504 (99.80%) polymorphic loci were detected, and 372 polymorphic loci (96.12%) were identified using 15 ISSR primers. At the species level, the S....
In this study, inter-simple sequence repeats (ISSRs) and sequence-related amplified polymorphism (SRAP) were applied to assess the genetic diversity in 38 species of Chrysanthemum and related genera. A total of 204 and 567 bands were amplified by 24 ISSR and 25 SRAP primers, of which 196 (97%) and 557 (99%) were polymorphic, respectively. The ISSR-based genetic similarity ranged from 0.016 to 0...
PURPOSE To investigate the significance of slowly rising abdominal pressure (SRAP), which is often observed in nonneurogenic children during bladder filling in video urodynamic studies (VUDSs). METHODS The records of patients who underwent VUDS from July 2011 to June 2013 were reviewed. SRAP was defined as a rising curve over 5 cm H2O from the baseline abdominal pressure during the filling ph...
A total of 100 Sporisorium scitamineum isolates were investigated by inter simple sequence repeat (ISSR) and single primer-sequence related amplified polymorphism (SP-SRAP) markers. These isolates were clearly assorted into three distinct clusters regardless of method used: either cluster analysis or by principal component analysis (PCA) of the ISSR, SP-SRAP, or ISSR + SP-SRAP data set. The tot...
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