Expression and purification of photosynthetic protein PsbR for crystallization The PsbR protein is a nuclear-encoded photosystem (PS) II subunit specifically found in higher plants, in particular this 10 kDa protein is a component of oxygen evolving complex (OEC) of PSII. The role of PsbR has not been clarified yet. It is reported that PsbR might have auxiliary role for the PsbP binding to PSII...

Anabaena Sensory Rhodopsin (ASR) stands out among the microbial retinal proteins in that, under light-adaptation (LA) conditions, it binds both the 13-cis isomer and the all-trans isomer of the protonated Schiff base of retinal (PSBR). In the dark-adapted (DA) state, more than 95% of the proteins bear all-trans PSBR, and the protein environment adopts a different equilibrium state. We report th...

Potential enhancement of intestinal neoplasia by compensatory mucosal hyperplasia was tested in rats subjected to 50% proximal small bowel resection (PSBR) 10 days after the last of 16 weekly injections of azoxymethane. Azoxymethane alone increased jejunal contents of RNA and DNA each by 26% at 17 to 18 weeks (p less than 0.01) before there was macroscopic evidence of neoplasia. Three months af...

Since pancreatic adaptation to massive proximal small bowel resection (PSBR) may be modulated through cholecystokinin (CCK) secretion, we tested the effect of the CCK antagonist CR-1409 on this response. Male Wistar rats (n = 72) weighing 220-225 g were randomised to receive either PSBR or transection/resuture followed by saline or CR-1409 (12 mg/kg daily subcutaneously). Rats were killed one, ...

Ancestors of vertebrates used ultraviolet vision. Some descendants preserved ultraviolet vision, whereas some others replaced it with violet vision, and then, some of avian lineages reinvented ultraviolet vision. Ultraviolet (absorption at ∼360 nm) and violet (410-440 nm) sensitivities of visual pigments are known to be affected by around 20 amino acid substitutions. The present quantum mechani...

One of the desiccation-tolerant mechanisms of the resurrection plant, Xerophyta humilis, is the ability to shut down photosynthesis reversibly. The X. humilis psbR and ChlP genes, encoding the 10 kDa polypeptide of photosystem II (PSII) and a geranylgeranyl reductase, respectively, were isolated in a differential display screen as dehydration-down-regulated and rehydration-up-regulated transcri...

BACKGROUND The performance of microalgal and bacterial systems devoted to wastewater treatment might be limited by the poor settling biomass inhibition nitrifying bacteria under high irradiance conditions. This work implemented a photo-sequencing batch bioreactor (PSBR) treating medium- low-strength wastewaters. impact short time on formation microalgal–bacterial aggregates (MABAs) was evaluate...

Atom transfer radical coupling (ATRC), performed with or without traps, has allowed for high extents of (Xc) a variety brominated polymers, yet structurally different polymeric chain ends require unique reagents and reaction conditions. Inspired by similar study that focused on universal conditions the controlled polymerization monomers using atom (ATRP), this work focuses developing single set...