1076 CLINICALCHEMISTRY, Vol. 33, No. 6, 1987 second, there was a large paraprotein band at 29 gIL in the beta region, which was determined to be an 1gM-kappa by immunofixation electrophoresis. To determine whether the presence of the paraprotein or an unusual property of this patient’s albumin was responsible for the inability to quantil albumin by bromcresol green binding, I isolated the patie...

both 2ME-treated and untreated samples. To allow us to compare the methods directly, we selected sera containing 1gM paraproteins, for which quantification in treated and untreated samples by E-SD was possible. Figure 1 shows the regression analysisbetween treated and untreated samples with 1gM paraproteins. The median concentration for untreated 1gM (mainly 19S) was 26.9 g/L (range 5.8.46.8 g/...

Pontet et al. (1) recently argued that, in contrast to polyclonal 1gM, accurate data on concentrations of monoclonal 1gM in different patients is difficult to obtain because of the dependence of the results on the technique. This statement is at variance with previously published work of De Bruijn and Klein (2, 3) who came to different conclusions. According to our findings, confirmed by Out et...

A commercial automated continuous-flow procedure for estimating specific serum proteins has been evaluated for use in determination of lgG, IgA, 1gM (both 19S and 7S), ai-antitrypsin, and cerebrospinal fluid proteins. Results of the automated procedure correlate well with manual methods, but are much more precise. Estimation of 7S 1gM by the automated method is more accurate than estimation by ...

Eighty patients with type 1 (insulin-dependent) diabetes (ages 0-39 years) were consecutively recruited by the Belgian Diabetes Registry. Sera obtained at clinically diagnosed onset (i.e., before start of insulin therapy or within 7 days of initial treatment) were analyzed for total 1gM concentrations and for 1gM binding to fixed rat splenocytes (lgM-LyAb) and permeabilized rat islet cells (lgM...

Both murine and human myeloma proteins have been shown to have antibodylike activity for serum lipoproteins. We studied a 58-yr-old woman (M.L.) with a lymphoma and a VDRL titer of 1:4096 and a nonreactive FTA-ABS. Serum electrophoresis revealed an M-component which was shown to be 1gM-kappa by immunoelectrophoresis and 2-mercaptoethanol treatment of the serum reduced the VDRL titer to 1:64. Im...

Manual procedures are presented for determining immunoglobulins IgG, IgA, and 1gM in human serum. The three immunoglobulins are quantitated, after reaction with specific ant sera, by measuring light scattering by the antigen-antibody complexes. The procedure is standardized by use of pooled human serum with known concentrations of IgG, IgA, and 1gM. Results with the present system correlated ex...

immunofixation (2), and beta-mercaptoethanol reduction (3) of serum, with subsequent zone electrophoresis (Figure 1). In each instance, such reduction caused the band to disappear. The immunofixation and immunoelectrophresis procedures confirmed the presence of polymeric, polyclonal 1gM. One would expect that a discrete band would still be present on zone electrophoresis after reduction if 1gM ...

Fifty-three cases of chronic lymphocytic leukemia (CIL) were studied for the presence of the B cell 1gM Fc receptor (Fc giR) using an aggregated 1gM reagent. Restricted surface immunoglobulin, using conventional immunofluorescent techniques and FACS analysis. was detected in 43 cases (81 %). The cells in the remaining ten cases (19%) expressed negligible surface immunoglobulin (sIgi and did not...

Nomifensine (Mental. Alival; Hoechst. Frankfurt. FRG). an antidepressant drug. may cause immune hemolytic anemia (IHA) of the so-called immune complex type that is believed to occur by means of an innocent-bystander mechanism. In this report we describe findings that are not consistent with this mechanism in a patient with nomifensine-induced intravascular IHA associated with renal failure. In ...