28 The ability of bifidobacteria to adhere to the intestine of human host is considered to be 29 important for efficient colonization and achieving probiotic effects. Bifidobacterium 30 bifidum strains DSM20456 and MIMBb75 adhere well to human intestinal cell lines, 31 Caco-2 and HT-29. Surface lipoprotein BopA has previously been described to be 32 involved in mediating adherence of B. bifidum...

A gene coding for a multicopper oxidase (BopA) was identified through the screening of a metagenomic library constructed from wastewater treatment activated sludge. The recombinant BopA protein produced in Escherichia coli exhibited oxidation activity toward 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) in the presence of copper, suggesting that BopA is laccase. A bioinformatic ana...

Autophagy is an important defense mechanism targeting intracellular bacteria to restrict their survival and growth. On the other hand, several intracellular pathogens have developed an antiautophagy mechanism to facilitate their own replication or intracellular survival. Up to now, no information about the origin or evolution of the antiautophagic genes in bacteria is available. BopA is an effe...

BACKGROUND Bifidobacteria belong to one of the predominant bacterial groups in the intestinal microbiota of infants and adults. Several beneficial effects on the health status of their human hosts have been demonstrated making bifidobacteria interesting candidates for probiotic applications. Adhesion of probiotics to the intestinal epithelium is discussed as a prerequisite for colonisation of a...

We found that the human intestinal isolate Bifidobacterium bifidum MIMBb75 strongly adhered to Caco-2 cells. Proteinase K and lithium chloride treatments showed that proteins play a key role in MIMBb75 adhesion to Caco-2 cells. By studying the cell wall-associated proteins, we identified a surface protein, which we labeled BopA. We purified the protein chromatographically and found that it func...

Calcium complexes bearing bis(oxazolinylphenyl)amine (BOPA) ligands have been prepared, and their redistribution chemistry studied. The complexes [Ca(R-BOPA){N(SiMe(3))(2)}(THF)] (R = (i)Pr 1a, Ph 1b and Bn 1c) are active in hydroamination catalysis and give the highest enantioselectivity reported for a calcium complex.

Peer assessment is the most common approach to evaluating scientific work, and it is also gaining popularity for scaling evaluation of student work in large and distributed classes. The key idea is that each peer reviewer or grader rates a relatively small subset of the items, and that some method of manual, semiautomatic, or fully-automatic aggregation of all assessments defines the eventual r...