An important level at which the expression of programmed cell death (PCD) genes is regulated is alternative splicing. Our previous work identified an intronic splicing regulatory element in caspase-2 (casp-2) gene. This 100-nucleotide intronic element, In100, consists of an upstream region containing a decoy 3' splice site and a downstream region containing binding sites for splicing repressor ...

In100, a new integron carrying a carbapenemase gene (bla(VIM-2)) associated with a carbenicillinase (blaP1b) and aminoglycoside resistance genes (aacA4 and aadA2), was detected in a Pseudomonas aeruginosa clinical isolate. The particular gene cassette organization of In100 seems to reflect the evolution of antibiotic usage in therapeutics.

The mean and the life-limiting behavior under fatigue of the nickel-based superalloy, IN100, separated (or converged) as a function of stress level and dwell loading. This behavior was related to the control of the life-limiting behavior by the smallcrack growth regime, producing its much slower response to stress level and dwell-time, relative to the mean-lifetime behavior. The lifetime probab...

We have been using the caspase-2 pre-mRNA as a model system to study the importance of alternative splicing in the regulation of programmed cell death. Inclusion or skipping of a cassette-type exon in the 3' portion of this pre-mRNA leads to the production of isoforms with antagonistic activity in apoptosis. We previously identified a negative regulatory element (In100) located in the intron do...

We have established a model system using the caspase-2 pre-mRNA and initiated a study on the role of alternative splicing in regulation of programmed cell death. A caspase-2 minigene construct has been made that can be alternatively spliced in transfected cells and in nuclear extracts. Using this system, we have identified a 100-nt region in downstream intron 9 that inhibits the inclusion of th...

The pharmacovigilance study was conducted in100 patients with pulmonary tuberculosis admitted pulmonology ward of Government general hospital, Anantapur, Andhra Pradesh. The purpose of this study is to assess the rate of adverse drug reactions induced by antitubercular drugs and also to detect serious and preventable ADRs.

Titanium and nickel alloys represent a significant metal portion of the aircraft structural and en components and the residual stresses induced by machining are very critical due to safety sustainability concerns. This paper presents experimental investigations and finite element simulat on turning of Ti–6Al–4V titanium alloy and IN100 nickel based alloy with uncoated and TiAlN co tools. Face t...

A three-dimensional (3-D) dataset of Ni-based superalloy Inconel 100 is used as a validation case for using stereology to estimate 3-D grain sizes from 2-D data. 2-D sections of the IN100 dataset are extracted, from which 3-D size distributions are estimated through the use of the Saltykov method and compared to the true 3-D statistics. The Saltykov method corrected the upper tail disparity bet...