Changes in HeLa cell morphology, membrane permeability, and viability caused by the presence of Triton X-100 (TX100), a nonionic surfactant, were studied by scanning electrochemical microscopy (SECM). No change in membrane permeability was found at concentrations of 0.15 mM or lower during an experimental period of 30 to 60 min. Permeability of the cell membrane to the otherwise impermeable, hi...

Surface tension, density and conductivity measurements were carried out for systems containing mixtures of cetyltrimethylammonium bromide CTAB) and p-(1,1,3,3-tetramethylbutyl)phenoxypoly(ethylene glycol), Triton X-100 (TX100). The obtained results of the surface tension meaurements were compared with those calculated from the relations derived by Joos, Miller and co-workers. From the compariso...

phase domains, leaving the lo-phase domains relatively unchanged as DRMs. Both of these approaches showed that TX100 did not substantially change the relative amounts of membrane in the two phases. By contrast, using calorimetry, Heerklotz and colleagues reported that TX100 can induce phase separation in previously uniform mixtures (45, 46). However, results of the other two studies described a...

phase domains, leaving the lo-phase domains relatively unchanged as DRMs. Both of these approaches showed that TX100 did not substantially change the relative amounts of membrane in the two phases. By contrast, using calorimetry, Heerklotz and colleagues reported that TX100 can induce phase separation in previously uniform mixtures (45, 46). However, results of the other two studies described a...

Electrophoretic light scattering, dynamic light scattering, turbidity, and static light scattering were used to study complex formation between poly(dimethyldiallylammonium chloride) (PDMDAAC) and oppositely charged mixed micelles of Triton X-100 (TX100) and sodium dodecyl sulfate (SDS) in 0.4 M NaC1. Interpolymer complexes form with an increasing bulk weight ratio of PDMDAAC to TX100-SDS, W, a...

phase domains, leaving the lo-phase domains relatively unchanged as DRMs. Both of these approaches showed that TX100 did not substantially change the relative amounts of membrane in the two phases. By contrast, using calorimetry, Heerklotz and colleagues reported that TX100 can induce phase separation in previously uniform mixtures (45, 46). However, results of the other two studies described a...

The di-tripeptide transport system (DtpT) of Lactococcus lactis was purified to apparent homogeneity by pre-extraction of crude membrane vesicles with octaethylene glycol monodecyl ether (C10E8), followed by solubilization with n-dodecyl-beta-D-maltoside (DDM) and chromatography on a Ni-NTA resin. The DtpT protein was reconstituted into detergent-destabilized preformed liposomes prepared from E...