Isolation of BCoV was performed in monolayers of HmLu-1 cells, using 20 fecal samples from clinical cases of diarrhea in calves. Samples were positive for BCoV by means of hemagglutination / hemagglutination inhibition (HA/HI). Up to the fifth serial passage, 13 of these isolates presented syncytial cytopathic effect, similar to Kakegawa standard strain. When isolates were submitted to seroneut...

Primary bovine embryonic kidney cell was successfully replaced by the green monkey cell line (Vero) for the isolation and seroneutralization of rinderpest virus.

We conducted a survey in Cambodia in 2000 on henipavirus infection among several bat species, including flying foxes, and persons exposed to these animals. Among 1,072 bat serum samples tested by enzyme-linked immunosorbent assay, antibodies reactive to Nipah virus (NiV) antigen were detected only in Pteropus lylei species; Cynopterus sphinx, Hipposideros larvatus, Scotophilus kuhlii, Chaerepho...

In order to study the seroprevalence of pestivirus infection in small ruminants of Ahvaz at Khouzestan province of Iran, 148 sheep sera and 143 goat sera were randomly collected and tested by seroneutralization. Seroneutralization was performed by NADL strain of Bovine viral diarrhea virus genotype 1. The results indicated the overall seroprevanelces of 46.62% in sheep and 32.871% in goats. Pre...

To determine whether West Nile virus (WNV) had reached the archipelago of Guadeloupe, a serologic study in horses and birds was conducted in 2002. Immunoglobulin (Ig) G, IgM, enzyme-linked immunosorbent assay, and seroneutralization tests identified WNV infection in horses and chickens. Six months later, a high rate of seroconversion was observed in horses.

Reemergence of Schmallenberg virus (SBV) occurred among lambs (n = 50) in a sheep flock in Belgium between mid-July and mid-October 2012. Bimonthly assessment by quantitative reverse transcription PCR and seroneutralization demonstrated that 100% of lambs were infected. Viremia duration may be longer in naturally infected than in experimentally infected animals.

in order to study the seroprevalence of pestivirus infection in small ruminants of ahvaz at khouzestan province of iran, 148 sheep sera and 143 goat sera were randomly collected and tested by seroneutralization. seroneutralization was performed by nadl strain of bovine viral diarrhea virus genotype 1. the results indicated the overall seroprevanelces of 46.62% in sheep and 32.871% in goats. pre...

Aiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin titers. Titers observed during in vivo and in vitro seroneutralization had a correlation of 99.12%, indicating that cell culture is a viab...

In the present study, six diagnostic methods for the detection of infectious pancreatic necrosis virus (IPNV) (indirect immunofluorescence, flow cytometry, immunoperoxidase, immunodot blot, immunostaphylococcus-protein A, and RT-PCR) have been comparatively evaluated using the seroneutralization as the reference assay, and 83 Spanish isolates and 3 reference strains. The most reliable methods w...