C-phycocyanin is a natural blue dye extracted from Spirulina platensis, which has many applications in the food and pharmaceutical industries. In this paper the effect of pH and temperature on the adsorption of C-phycocyanin onto two different ion exchange resins (Streamline DEAE and Streamline Q XL) for expanded bed adsorption chromatography was investigated. Moreover, the kinetics and adsorpt...

Abstract A chemical modification of cotton fabrics by 2-diethylaminoethyl chloride (DEAE-Cl) was achieved, and the resulted demonstrated salt-free dyeing properties with anionic dyes. Nucleophilic property hydroxyl groups in cellulose enhanced under alkaline conditions could react DEAE-Cl, a possessing both nucleophilic electrophilic sites. The monolayered DEAE-grafted further DEAE-Cl to form m...

Expanded bed adsorption (EBA) is an integration technology for capturing target bioproducts directly from unclarified feedstock, designing and applying a good adsorbent affects its performance. In present work, a novel matrix has been developed for expanded bed adsorption process. The prepared matrix was manufactured using water-in-oil emulsification method. Desirable adsorbents tend to be of s...

The enzyme inulinase is used to produce oligosaccharides and fructose, with up to 95% fructose in a single stage of inulina hydrolysis. With in the aim to purify the enzyme, studies on the conditions of enzyme adsorption in an expanded-bed column were conducted using phosphate and tris-HCl buffers. The inulinase used in this work was obtained from Kluyveromyces marxianus NRRL Y-7571 by fermenta...

A complete study of thermophysical properties concerning the 2-(2-diethylaminoethoxy)ethanol (DEAE-EO) + water binary system is realized. Density, speed sound, dynamic and kinematic viscosities refractive index measurements have been performed at atmospheric pressure, using a vibrating tube densitometer, falling ball viscosimeter, refractometer for pure DEAE-EO, water, aqueous solutions from 27...

Aqueous extracts of seeds of Duranta plumieri were found to be rich in polyphenol oxidase activity. The anion-exchange chromatography of the crude extract on Streamline DEAE resolved the activity into three fractions. The major fraction (77% of the total activity) was further purified by treating it with concanavalin A-agarose in the batch mode. The enzyme preparation eluting with alpha-methylm...