The Drosophila melanogaster gene Sgs-1 belongs to the secretion protein genes, which are coordinately expressed in salivary glands of third instar larvae. Earlier analysis had implied that Sgs-1 is located at the 25B2-3 puff. We cloned Sgs-1 from a YAC covering 25B2-3. Despite using a variety of vectors and Escherichia coli strains, subcloning from the YAC led to deletions within the Sgs-1 codi...

RESULTS: The mean SEI, fibroblast counts, and capillary counts differed significantly among the five groups (P < 0.05). The median SEI was significantly lower in the SGS+2.5 mg verapamil/g group (Figure 1) than in the SGS group (Figure 2) (1.2 vs. 2.2). The median number of fibroblasts was significantly lower in the SGS+0.25 mg verapamil/g group than in the SGS group (172.5 vs. 243). The median...

Insulin secretion is key for glucose homeostasis. Insulin secretory granules (SGs) exist in different functional pools, with young SGs being more mobile and preferentially secreted. However, the principles governing the mobility of age-distinct SGs remain undefined. Using the time-reporter insulin-SNAP to track age-distinct SGs we now show that their dynamics can be classified into three compon...

Spatial Genetic Structure (SGS) is correlated with life history traits and is affected by ecological and demographic processes. We discuss here the SGS within populations of three species from Cerrado Biome, Caryocar brasiliense, Dipteryx alata and Tibouchina papyrus, which vary in life history traits. Our working hypothesis was that self-incompatible and wind-dispersed species present no SGS d...

Stress granules (SGs) are cytoplasmic bodies wherein translationally silenced mRNAs are recruited for triage in response to environmental stress. We report that Drosophila cells form SGs in response to arsenite and heat shock. Drosophila SGs, like mammalian SGs, are distinct from but adjacent to processing bodies (PBs, sites of mRNA silencing and decay), require polysome disassembly, and are in...

Stress granules (SGs) are cytoplasmic RNA multimeric bodies that form under stress conditions known to inhibit translation initiation. In most reported stress cases, the formation of SGs was associated with the cell recovery from stress and survival. In cells derived from cancer, SGs formation was shown to promote resistance to either proteasome inhibitors or 5-Fluorouracil used as chemotherape...

Cells form stress granules (SGs) in response to environmental stresses, which constitute cytoplasmic domains where mRNAs are stored and translation is halted. Although several components are found in SGs, it is poorly understood as to how SGs are formed and dissolved. We identified growth factor receptor-bound protein 7 (Grb7), an RNA-binding, translational regulator, as an integral component o...