نتایج جستجو برای: تست dna unwinding

تعداد نتایج: 524161  

ژورنال: :مجله علوم و فنون دریایی 2015
احمد نگین تاجی بیتا ارچنگی عبدالعلی موحدی نیا علیرضا صفاهیه غلامرضا اسکندری

در این مطالعه، اثر bpaبر القای ناهنجاری های هسته ای و تخریب dna کبدی (روشdna unwinding assay ) در جنس نر ماهی شانک زردباله (acanthopagrus latus) مورد بررسی قرار گرفته است. ماهیان طی دو هفته و به صورت تزریق درون صفاقی، غلظت های 10، 50، 100 و 150 میکروگرم بر گرم وزن بدن ماهیان از bpa را به صورت محلول در روغن نارگیل دریافت نمودند. گروه کنترل حلال، تنها روغن نارگیل را دریافت کردند در حالیکه برروی م...

Journal: :Nucleic acids research 1993
A Georgaki U Hübscher

Replication protein A (RP-A) is a heterotrimeric single-stranded DNA binding protein with important functions in DNA replication, DNA repair and DNA recombination. We have found that RP-A from calf thymus can unwind DNA in the absence of ATP and MgCl2, two essential cofactors for bona fide DNA helicases (Georgaki, A., Strack, B., Podust, V. and Hübscher, U. FEBS Lett. 308, 240-244, 1992). DNA u...

Journal: :Nature Reviews Molecular Cell Biology 2009

Journal: :Journal of molecular biology 2002
Aaron L Lucius Alessandro Vindigni Razmic Gregorian Janid A Ali Andrew F Taylor Gerald R Smith Timothy M Lohman

The mechanism by which Escherichia coli RecBCD DNA helicase unwinds duplex DNA was examined in vitro using pre-steady-state chemical quenched-flow kinetic methods. Single turnover DNA unwinding experiments were performed by addition of ATP to RecBCD that was pre-bound to a series of DNA substrates containing duplex DNA regions ranging from 24 bp to 60 bp. In each case, the time-course for forma...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1990
G T Runyon D G Bear T M Lohman

The Escherichia coli uvrD gene product, helicase II, is required for both methyl-directed mismatch and uvrABC excision repair and is believed to function by unwinding duplex DNA. Initiation of unwinding may occur specifically at either a mismatch or a nick, although no direct evidence for this has previously been reported. It has recently been shown that helicase II can unwind fully duplex line...

2012
Jose A. Morin Francisco J. Cao José M. Valpuesta José L. Carrascosa Margarita Salas Borja Ibarra

DNA replication requires overcoming the energetic barrier associated with the base pair melting of its double helix and a finetuned coordination between the processes of DNA unwinding and DNA replication. One intriguing question that remains poorly understood is the exact mechanism of the coupling of these two reactions. In some organisms, these activities are coupled within the same protein, l...

Journal: :Nucleic Acids Research 2006
Ilker Donmez Smita S. Patel

Bacteriophage T7 helicase (T7 gene 4 helicase-primase) is a prototypical member of the ring-shaped family of helicases, whose structure and biochemical mechanisms have been studied in detail. T7 helicase assembles into a homohexameric ring that binds single-stranded DNA in its central channel. Using RecA-type nucleotide binding and sensing motifs, T7 helicase binds and hydrolyzes several NTPs, ...

2013
Liisa T. Chisty Christopher P. Toseland Natalia Fili Gregory I. Mashanov Mark S. Dillingham Justin E. Molloy Martin R. Webb

The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the oligomeric state of the active helicase complex, its processivity and the mechanism of unwinding. By tethering either DNA or PcrA to a microscop...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2012
José A Morin Francisco J Cao José M Lázaro J Ricardo Arias-Gonzalez José M Valpuesta José L Carrascosa Margarita Salas Borja Ibarra

Duplication of double-stranded DNA (dsDNA) requires a fine-tuned coordination between the DNA replication and unwinding reactions. Using optical tweezers, we probed the coupling dynamics between these two activities when they are simultaneously carried out by individual Phi29 DNA polymerase molecules replicating a dsDNA hairpin. We used the wild-type and an unwinding deficient polymerase varian...

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