Inhibition of dipeptidyl peptidase-IV (DPP-IV) is used as a means to regulate post-prandial serum glucose in type 2 diabetics. The effect of drug (Sitagliptin®)/peptide and binary peptide mixtures on DPP-IV inhibition was studied using an isobole approach. Five peptides (Ile-Pro-Ile-Gln-Tyr, Trp-Lys, Trp-Pro, Trp-Arg and Trp-Leu), having DPP-IV half maximum inhibitory concentration values (IC₅₀...

The amino acid sequence of Chromatium ferredoxin was determined by studies of thermolysin peptides of S-carboxymethylcysteinylferredoxin and chymotryptic peptides of oxidized ferredoxin. The 81 amino acid residues in the molecule have the following sequence: Ala-Leu-Met-IleThr-Asp-Gln-Cys-Ile-Asn-Cys-Asn-Val-Cys-Gln-ProGlu-Cys-Pro-Asn-Gly-Ala-Ile-Ser-Gln-Gly-Asp-Glu-ThrTyr-Val-Ile-Glu-Pro-Ser-L...

The amino acid sequences of both the alpha and beta subunits of human chorionic gonadotropin have been determined. The amino acid sequence of the alpha subunit is: Ala - Asp - Val - Gln - Asp - Cys - Pro - Glu - Cys-10 - Thr - Leu - Gln - Asp - Pro - Phe - Ser - Gln-20 - Pro - Gly - Ala - Pro - Ile - Leu - Gln - Cys - Met - Gly-30 - Cys - Cys - Phe - Ser - Arg - Ala - Tyr - Pro - Thr - Pro-40 -...

The primary structure of cholera toxin B subunit, responsible for the binding of the toxin to cell surface ganglioside Gm1, has been determined as: Thr-Pro-Gln-Asn-Ile-Thr-Asp-Leu-Cys-Ala-Glu-Tyr-His-Asn-Thr-Gln-Ile-His-Thr-Leu-Asn-Asn-Lys-Ile-Phe-Ser-Tyr-Thr-Glu-Ser-Leu-Ala-Gly-Lys-Arg-Glu-Met-Ala-Ile-Ile-Thr-Phe-Lys-Asn-Gly-Ala-Thr-Phe-Glu-Val-Glu-Val-Pro-Gly-Ser-Gln-His-Ile-Asp-Ser-Gln-Lys-L...

2 Ser Asn Asn Asn 14 Leu Ile Leu Leu 22 Ser Ser Leu Leu 31 Val Asp Val Val 67 Thr Ala Thr Ala 116 Val Ile Ile Ile 183 Ala Ala Glu Glu 210 Ala Ser Ser Ser 233 Val Ala Ala Ala 262 Thr Ile Thr Thr 277 Gln Gln Arg Gln 294 Thr Val Val Val 296 Pro Ser Ser Ser 305 Ala Ser Ser Ser 314 Ile Val Val Val 317 Glu Lys Lys Lys 378 Ile Leu Leu Leu 415 Leu Val Val Val 417 Asp Gly Gly Gly 421 Val Ala Ala Ala 432...

Peptides having potent antioxidant activity were separated from the hydrolysate of wheat gluten by ion-exchange and gel filtration chromatography. Peptides obtained by SP Sephadex C-25 chromatography of the most active fraction (WP-3) were further separated using reversed-phase high performance liquid chromatography. The amino acid sequences of these peptides were Leu-Gln-Pro-Gly-Gln-Gly-Gln-Gl...

The following sequences of amino acids surrounding the three sulfhydryl groups of the Escherichia coli tryptophan synthetase A protein were established: Sequence I, Gly-IleAsp-Glu-Phe-Tyr-Ala-Gln-Cys-Glu-Lys-Val-Gly-ValAsp-Ser-Val-Leu-Val-Ala-Asp-Val-Gln-Glu-Ser-Ala-ProPhe-Arg; Sequence II, Arg-Ala-Phe-Ala-Ala-Gly-Val-ThrPro-Ala-Gln-Cys-Phe-Glu-Met-Leu-Ala-Leu-Ile-Arg; Sequence III, Arg-His-Asn...

Phospholamban is a regulatory protein in cardiac sarcoplasmic reticulum that is phosphorylated by cAMP- and Ca2+/calmodulin-dependent protein kinase activities. In this report, we present the partial amino acid sequence of canine cardiac phospholamban and the identification of the sites phosphorylated by these two protein kinases. Gas-phase protein sequencing was used to identify 20 NH2-termina...

Rabbit brain endo-oligopeptidase B inactivates angiotensin I (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu) and angiotensin II (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe) by hydrolysis of the Pro7-Phe8 peptide bond. The site of hydrolysis was determined in preparative and analytical experiments in which both products were recovered in a molar ratio of 1:1, and the sum of the products plus unhydrolyzed substra...