Chicken egg yolk antibodies (IgY) against camel immunoglobulins were generated and their specificity was shown by double immunodiffusion, immunoelectrophoresis, and Western blotting. Anti camel antibodies specifically react to camel antibodies in immunodiffusion and immunoelectrophoresis were demonstrated. Western blotting revealed that IgY antibodies recognize the heavy chain in a number of ma...

On examination of Bactec blood cultures for pneumococcal antigen by countercurrent immunoelectrophoresis the consistent presence of a non-specific protein band caused problems with the interpretation of results, even in laboratories experienced in performing countercurrent immunoelectrophoresis. When the Bactec blood culture system is used, countercurrent immunoelectrophoresis should not be rel...

background : brucellosis is a major bacterial zoonosis of global importance with the causative organisms of gram-negative facultative intracellular pathogens. the aims of this study were to standardize two immunoelectrophoretic techniques, rocket and cross immunoelectrophoresis, and compare their results with other conventional serodiagnostic tests. methods : sera from 15 sheep, without any his...