poly-β-hydroxybutyrate (phb), produced by several species of bacteria, has attracted great attention as a biodegradable and biocompatible compound with almost similar properties of polypropylene. unfortunately, its use is currently limited due to high production costs. one of the most common methods for overcoming this constraint is the use of inexpensive substrates, like methanol. another stra...

In the current study, the effect of dietary supplementation of poly-β-hydroxybutyrate (PHB) on growth performance, digestive enzymes activity, body composition, non-specific immune response and diseases resistance of rainbow trout fingerlings were investigated. Five hundred and fifty-two healthy fingerlings (8.2±1.0 g) were randomly distributed in 12 tanks (150 L) at a density of 46 fish/ tank ...

Ralstonia eutropha H16 degraded (mobilized) previously accumulated poly(3-hydroxybutyrate) (PHB) in the absence of an exogenous carbon source and used the degradation products for growth and survival. Isolated native PHB granules of mobilized R. eutropha cells released 3-hydroxybutyrate (3HB) at a threefold higher rate than did control granules of nonmobilized bacteria. No 3HB was released by n...

Identification of proteins that were present in a polyhydroxybutyrate (PHB) granule fraction isolated from Ralstonia eutropha but absent in the soluble, membrane, and membrane-associated fractions revealed the presence of only 12 polypeptides with PHB-specific locations plus 4 previously known PHB-associated proteins with multiple locations. None of the previously postulated PHB depolymerase is...

BACKGROUND Poly-beta-hydroxybutyrate (PHB) mobilization in bacteria has been proposed as a mechanism that can benefit their host for survival under stress conditions. Here we reported for the first time that a stress-induced system enabled E. coli, a non-PHB producer, to mobilize PHB in vivo by mimicking natural PHB accumulation bacteria. RESULTS The successful expression of PHB biosynthesis ...

Herbaspirillum seropedicae is a diazotrophic ß-Proteobacterium found associated with important agricultural crops. This bacterium produces polyhydroxybutyrate (PHB), an aliphatic polyester, as a carbon storage and/or source of reducing equivalents. The PHB polymer is stored as intracellular insoluble granules coated mainly with proteins, some of which are directly involved in PHB synthesis, deg...

Considering the industrial interest of biodegradable polymer poly-3-hydroxybutyrate (PHB), the marine bacteria Neptunomonas antarctica was studied for its ability to accumulate PHB. The extracted polymer was confirmed to be PHB by nuclear magnetic resonance analysis. In shake flask cultures using natural seawater as medium components, PHB was produced up to 2.12 g/L with a yield of 0.18 g PHB/g...