نتایج جستجو برای: آگار 7h10 .

تعداد نتایج: 2693  

ژورنال: :مجله تحقیقات نظام سلامت حکیم 0
دکتر محمد نجفی مصلح najafimosleh m ,(phd); همدان، خیابان مهدیه، دانشکده پزشکی، گروه میکروبیولوژی، کد پستی 65155 تلفن: 09188131189 نمابر: 8276299-0811 دکترکیومرث قاضی سعیدی ghazi-saiedi k, (phd); دکتر پریسا فرنیا farnia p, (phd); دکتر فروزان محمدی mohammadi f, (md); دکترعلی اکبر ولایتی velayati aa, (md).

مقدمه: پیرازینامید یکی از چهار داروی خط اول ضدسلی است که علاوه بر کوتاه کردن دوره درمان با از بین بردن اشکال فعال و غیرفعال باسیل سل در محیط اسیدی درون ماکروفاژها، در ریشه کن کردن ارگانیسم در بدن بیمار بسیار مؤثر است. بر خلاف سایر داروهای ضدسلی فعال بودن پیرازینامید در ph اسیدی (در صورتی که ph مناسب برای رشد ارگانیسم 8/6 می باشد)، سنجش حساسیت باسیل سل به این دارو را با مشکل اساسی مواجه ساخته اس...

Journal: :Applied microbiology 1973
P I Liu D H McGregor I Faucher W L Jinks L A Miller L Green J G Liu

Among 2,648 specimens positive on culture for Mycobacterium tuberculosis over a 6-year period, 82% grew on Lowenstein-Jensen medium (LJ), 79% on American Trudeau Society (ATS), and 56% on Middlebrook 7H10 (7H10). When these commercial culture media were compared in regard to the number of acid-fast bacilli seen on the original smears, LJ cultures were found to have the highest isolation rates f...

Journal: :Applied microbiology 1967
V Lorian

In a comparison of Lowenstein's medium with two agar media, 7H10 without and with the addition of Triton WR 1339, 4,100 sputa were planted on all three, yielding 803 (19.6%) positive cultures. The addition of Triton WR 1339 to the 7H10 medium made it possible to observe the cord formation directly on the isolation plates and did not alter, in any respect, the results obtained with the basic 7H1...

Journal: :Journal of clinical microbiology 1979
C O Thoen E M Himes J L Jarnagin R Harrington

The efficiency of four culture media was compared for the isolation of Mycobacterium avium complex from 197 procine tissues. In 82 tissues with microscopic granulomas and acid-fast bacilli, a significantly greater number of isolates were obtained on Middlebrook 7H10 medium with sodium pyruvate than on Stonebrink medium, Herrold egg yolk agar medium, or Lowenstein-Jensen medium (P=0.01). In 46 t...

Journal: :Journal of clinical microbiology 1983
H Takahashi V Foster

During a 6-month period, 5,375 clinical specimens were cultured on Middlebrook-Cohn 7H10 medium, on Lowenstein-Jensen medium, and in Middlebrook 7H12 medium containing [14C]palmitic acid. More mycobacteria were recovered when all three media were used than when either the conventional method with 7H10 agar and Lowenstein-Jensen slants or the radiometric method with 7H12 broth was used alone.

دکتر فروزان محمدی, , دکتر محمد نجفی مصلح, , دکتر پریسا فرنیا, , دکترعلی‌اکبر ولایتی, , دکترکیومرث قاضی‌سعیدی, ,

مقدمه: پیرازینامید یکی از چهار داروی خط اول ضدسلی است که علاوه بر کوتاه کردن دوره درمان با از بین بردن اشکال فعال و غیرفعال باسیل سل در محیط اسیدی درون ماکروفاژها، در ریشه‌کن کردن ارگانیسم در بدن بیمار بسیار مؤثر است. بر خلاف سایر داروهای ضدسلی فعال بودن پیرازینامید در pH اسیدی (در صورتی که pH مناسب برای رشد ارگانیسم 8/6 می‌باشد)، سنجش حساسیت باسیل سل به این دارو را با مشکل اساسی مواجه ساخته اس...

Journal: :Journal of clinical microbiology 2016
Koné Kaniga Daniela M Cirillo Sven Hoffner Nazir A Ismail Devinder Kaur Nacer Lounis Beverly Metchock Gaby E Pfyffer Amour Venter

The aim of this study was to establish standardized drug susceptibility testing (DST) methodologies and reference MIC quality control (QC) ranges for bedaquiline, a diarylquinoline antimycobacterial, used in the treatment of adults with multidrug-resistant tuberculosis. Two tier-2 QC reproducibility studies of bedaquiline DST were conducted in eight laboratories using Clinical Laboratory and St...

Journal: :Journal of clinical microbiology 2005
Ahmet Yilmaz Coban Kemal Bilgin Meltem Uzun Nuriye Tasdelen Fisgin Alper Akgunes Cigdem Cekic Cihan Asuman Birinci Belma Durupinar

In this study, blood agar was used instead of 7H10 agar for the susceptibility testing of 34 clinical isolates of Mycobacterium tuberculosis to isoniazid (INH) and rifampin (RIF) in accordance with the NCCLS. The BACTEC 460 TB system (Becton Dickinson, Sparks, Md.) was used as a "gold standard." Results for both media were in agreement for RIF and INH at 100 and 94.1%, respectively. For INH, th...

Journal: :Journal of clinical microbiology 1983
J J Damato M T Collins M V Rothlauf J K McClatchy

A group of 89 smear-positive sputum specimens were evaluated by radiometric and standard plate procedures to determine the methodology which would provide the earliest detection of mycobacteria and maximum test sensitivity. Digested non-decontaminated specimens were concentrated and inoculated into modified selective BACTEC radiometric 7H12 broth and Mitchison selective 7H10 agar. Sodium hydrox...

Journal: :Antimicrobial agents and chemotherapy 1975
J R Bonalsky D Jaconia E A Konopka F W Adair

Rifampin was incorporated into Middlebrook 7H10 medium either by adding an aliquot of the antibiotic into melted agar (final concentration 1.0 and 3.0 mug/ml) or by submerging a 5 or 15 mug of rifampin paper disk into 5 ml of melted agar contained in one quadrant of a Felson "X" plate. At intervals, plugs of agar were removed from the stored plates and assayed. Plates stored at 5 C for 28 days ...

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