some kinetic properties of nad+- and nadp+- dependent glucose 6-phosphatedehydrogenase (g6pd) purified from streptomyces aureofaciens were studied. both nadh andnadph inhibited the enzyme competitively and noncompetitively, with respect to the correspondingcoenzymes and glucose 6-phosphate, respectively. atp inhibited the nad+ - linked reaction but not thatof the nadp+- linked activity. the inh...

Hier berichten wir über ein neues robustes Nicotinamid-Dinukleotidphosphat-Cofaktor-Analogon (carba-NADP+) und dessen Akzeptanz von zahlreichen Enzymen aus der Klasse Oxidoreduktasen. Es wurde festgestellt, dass das Ersetzen eines Ribose-Sauerstoffs durch eine Methylengruppe des natürlichen NADP+ die Stabilität drastisch erhöht. Zersetzungsexperimente bei moderaten hohen Temperaturen mit den Co...

Several microorganisms can utilize l-rhamnose as a carbon and energy source through the nonphosphorylative metabolic pathway, in which 1-dehydrogenase (RhaDH) catalyzes NAD(P)+-dependent oxidization of to l-rhamnono-1,4-lactone. We herein investigated crystal structures RhaDH from Azotobacter vinelandii ligand-free, NAD+-bound, NADP+-bound, l-rhamnose- NAD+-bound forms at 1.9, 2.1, 2.4, 1.6 Å r...

interaction of glucose 6-phosphate dehydrogenase from s. aureofaciens with nad+, nadp+and glucose 6-phosphate were investigated using different fluorescent probes. binding of nad+, nadp+and s-nadph to the native enzyme quenched intrinsic protein fluorescence by 100%, 10% and 21%,respectively, from which kd values of nad+ (6.5 mm), nadp+ (92.0 μm) and s-nadph (122.0 μm)were calculated. binding o...

The Arabidopsis (Arabidopsis thaliana) genome contains four genes encoding putative NADP-malic enzymes (MEs; AtNADP-ME1-ME4). NADP-ME4 is localized to plastids, whereas the other three isoforms do not possess any predicted organellar targeting sequence and are therefore expected to be cytosolic. The plant NADP-MEs can be classified into four groups: groups I and II comprising cytosolic and plas...

Purified potato tuber (Solanum tuberosum L. cv Bintie) mitochondria contain soluble, highly latent NAD(+)- and NADP(+)-isocitrate dehydrogenases, NAD(+)- and NADP(+)-malate dehydrogenases, as well as an NADPH-specific glutathione reductase (160, 25, 7200, 160, and 16 nanomoles NAD(P)H per minute and milligram protein, respectively). The two isocitrate dehydrogenase activities, but not the two m...

In plants, the NADP malic enzymes (NADP-MEs) are encoded by small gene families. These NADP-ME gene families are relatively well described in C4 plants but not well studied in C3 plants. In this study, we investigated the NADP-ME gene family in a model C3 monocot plant (rice, Oryza sativa) based on its recently released genomic DNA sequence. We found that the rice NADP-ME family is composed of ...

The experiments were designed to get some information on the metabolism controlled by variation of the NADP level, which is known to change with the variation of environmental factors.The exogenous NADP added to the mitochondria prepared from Vigna sesquipedalis cotyledons was associated with and/or penetrated into the mitochondria. The combined NADP served in the operation of the mitochondrial...