In order to study the seroprevalence of pestivirus infection in small ruminants of Ahvaz at Khouzestan province of Iran, 148 sheep sera and 143 goat sera were randomly collected and tested by seroneutralization. Seroneutralization was performed by NADL strain of Bovine viral diarrhea virus genotype 1. The results indicated the overall seroprevanelces of 46.62% in sheep and 32.871% in goats. Pre...

twelve (experiment i) and four (experiment ii) multiparous dairy cows seronegative to pestivirus were selected and randomly assigned to either a control group which did not become infected or a treatment group in which all cows became infected following intranasal inoculation 9 days before ai. the experimental induction of infection was carried out with 2 ml of non-cytopathic pestivirus (bvd vi...

twelve (experiment i) and four (experiment ii) multiparous dairy cows seronegative to pestivirus were selected and randomly assigned to either a control group which did not become infected or a treatment group in which all cows became infected following intranasal inoculation 9 days before ai. the experimental induction of infection was carried out with 2 ml of non-cytopathic pestivirus (bvd vi...

in order to study the seroprevalence of pestivirus infection in small ruminants of ahvaz at khouzestan province of iran, 148 sheep sera and 143 goat sera were randomly collected and tested by seroneutralization. seroneutralization was performed by nadl strain of bovine viral diarrhea virus genotype 1. the results indicated the overall seroprevanelces of 46.62% in sheep and 32.871% in goats. pre...

An outbreak of abortion affecting multiparous cows was associated with Hobi-like pestivirus infection. Viral RNA and antigens were detected in the tissues of two aborted fetuses. Molecular assays for other common abortogenic agents tested negative. At the genetic level, the Hobi-like pestivirus displayed the closest relatedness to Italian, Australian, and South American viruses, whereas it dive...

A calf persistently infected by Hobi-like pestivirus was monitored for about 6 months, displaying clinical signs typical of bovine viral diarrhea virus persistent infection and shedding the virus through all body secretions, with maximal titers detected in urine. This report provides new insights into the pathogenesis of the emerging pestivirus.

Pestivirus infections are commonly diagnosed in cattle but are relatively uncommon in other ruminant species in Australia. Virus isolation is a very reliable technique for detecting pestivirus in specimens, especially when group reactive monoclonal antibodies are used with immunoperoxidase staining to detect non-cytopathogenic virus. Care must be taken to prevent adventitious pestivirus contami...

We propose the creation of seven new species in the genus Pestivirus (family Flaviviridae) in addition to the four existing species, and naming species in a host-independent manner using the format Pestivirus X. Only the virus species names would change; virus isolates would still be referred to by their original names. The original species would be re-designated as Pestivirus A (original desig...

Pestiviruses initiate infection of susceptible cells by receptor-mediated endocytosis. Cellular plasma membrane or endosomal molecules involved in translocation of these viruses into the cytosol have not been unequivocally identified. We reported previously that a mutant cell line derived from Madin-Darby bovine kidney (MDBK) cells, termed CRIB-1, was resistant to infection with bovine viral di...

Ruminant pestivirus infections of pigs have a worldwide distribution. The prevalence is varied and depends mainly on (i) contact with cattle, (ii) age of pigs and (iii) degree of homology of virus strains used for serology, with field strains of bovine virus diarrhoea virus (BVDV) infecting pigs. Emphasis should be laid on sources of BVDV other than cattle, e.g. contaminated vaccines and fetal ...