The new colorimetric Vitek 2 YST card was evaluated for identification of yeasts (136 strains) with respect to the influence of different source media. The Vitek 2 YST card achieved satisfactory results for all yeast species tested, with the exception of Candida guilliermondii, Candida norvegensis, Candida parapsilosis, Candida rugosa, and Candida tropicalis. After simple additional tests, 93.7...

The new VITEK 2 system is a fully automated system dedicated to the identification and susceptibility testing of microorganisms. In conjunction with the VITEK ID-YST card the VITEK 2 system allows the identification of clinically important yeasts and yeast-like organisms in 15 h due to a sensitive fluorescence-based technology. The ID-YST card consists of 47 biochemical reactions. The database ...

Hill, Stephanie Rene. The Effects of Cottonseed Hulls Added to Diets With and Without Live Yeast or Mannanoligosaccharide in Young Calves (Under the direction of Dr. B. A. Hopkins and Dr. L. W. Whitlow). The objective of this study was to investigate the effects of fiber in the form of cottonseed hulls (CSH) added to the starter and of live yeast (YST) or mannanoligosaccharide (MOS) added to mi...

To produce rarely secreted recombinant proteins in the yeast Saccharomyces cerevisiae, we developed a novel genome-wide optimal translational fusion partner (TFP) screening system that involves recruitment of an optimal secretion signal and fusion partner. A TFP library was constructed from a genomic and truncated cDNA library by using the invertase-based signal sequence trap technique. The eff...

Yellow starthistle (YST) (Centaurea solstitialis) is an invasive weed in the USA and a target of classical biological control. However, the precise geographical origin(s) of YST in the USA is not known. This study was conducted to learn whether the origin(s) of YST in California, USA could be determined on the basis of digital image analysis (DIA) of YST reactions to isolates of the rust Puccin...

C is an increasingly common problem in hospitalized patients, especially among immunocompromised patients. C. albicans is the predominant cause of candidemia, with a crude mortality rate of approximately 40% [1-5]. However, non-albicans Candida species such as C. parapsilosis, C. glabrata, C. krusei and C. tropicalis are emerging as opportunistic pathogens [2]. C. parapsilosis is the second mos...

We constructed a Yst-negative mutant of Yersinia enterocolitica W1024 by reverse genetics, and we compared the virulence of the yst+ and yst isogenic strains in an experimental oral infection of the young rabbit. The rabbits infected with the yst+ strain suffered from the diarrhea and lost weight, and most of them died. By contrast, the occurrence of diarrhea, weight loss, and death in the grou...

Twenty-eight clinical isolates of Yersinia enterocolitica were investigated for their abilities to produce heat-stable enterotoxin (YST). All 21 invasive strains (serogroup O3 biotype 4) carried the previously described gene for YST (yst), with toxin detectable in culture supernatants from 20 strains. One of seven noninvasive, biotype 1A strains also had enterotoxic activity, despite failure to...

An in vitro gas production technique was used in this study to elucidate the effect of two strains of active live yeast on methane (CH4) production in the large intestinal content of pigs to provide an insight to whether active live yeast could suppress CH4 production in the hindgut of pigs. Treatments used in this study include blank (no substrate and no live yeast cells), control (no live yea...