نتایج جستجو برای: "nrpS"

تعداد نتایج: 574  

2001
Liangcheng Du Ben Shen

The structural and catalytic similarities between modular nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) inspired us to search for hybrid NRPS PKS systems. By examining the biochemical and genetic data known to date for the biosynthesis of hybrid peptide polyketide natural products, we show (1) that the same catalytic sites are conserved between the hybrid NRPS PKS and nor...

2016
Naing Ye Aung Rintaro Ohe Hongxue Meng Takanobu Kabasawa Suran Yang Tomoya Kato Mitsunori Yamakawa Ludovic Tailleux

In the immune system, neuropilins (NRPs), including NRP-1 and NRP-2, are expressed in thymocytes, dendritic cells, regulatory T cells and macrophages. Their functions on immune cells around the neoplastic cells vary into pro-angiogenesis, tumor progression and anti-angiogenesis according to their ligands. Even though NRPs expression on malignant tumors and immune system has studied, a PubMed-ba...

2017
B. Shen Liangcheng Du C. Sanchez D. J. Edwards M. Chen J. M. Murrell

The hybrid peptide–polyketide backbone of bleomycin (BLM) is assembled by the BLM megasynthetase that consists of both nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) modules. BlmIX/BlmVIII/BlmVII constitute a natural hybrid NRPS/PKS/NRPS system, serving as a model for both hybrid NRPS/PKS and PKS/NRPS systems. Sequence analysis and functional comparison of domains and modu...

Journal: :Journal of natural products 2002
Ben Shen Liangcheng Du Cesar Sanchez Daniel J Edwards Mei Chen Jeffrey M Murrell

Bleomycin (BLM) biosynthesis has been studied as a model for hybrid peptide-polyketide natural product biosynthesis. Cloning, sequencing, and biochemical characterization of the blm biosynthetic gene cluster from Streptomyces verticillus ATCC15003 revealed that (1) the BLM hybrid peptide-polyketide aglycon is assembled by the BLM megasynthetase that consists of both nonribosomal peptide synthet...

Journal: :Applied and environmental microbiology 2005
Xiaofeng Wu Johnathan Ballard Yi Wei Jiang

We isolated a novel gram-positive bacterium, Brevibacillus texasporus, that produces an antibiotic, BT. BT is a group of related peptides that are produced by B. texasporus cells in response to nutrient limitation. We report here purification and determination of the structure of the most abundant BT isomer, BT1583. Amino acid composition and tandem mass spectrometry experiments yielded a parti...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2014
Hao Wang David P Fewer Liisa Holm Leo Rouhiainen Kaarina Sivonen

Nonribosomal peptides and polyketides are a diverse group of natural products with complex chemical structures and enormous pharmaceutical potential. They are synthesized on modular nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) enzyme complexes by a conserved thiotemplate mechanism. Here, we report the widespread occurrence of NRPS and PKS genetic machinery across the thr...

Journal: :Nucleic acids research 2004
Mohd. Zeeshan Ansari Gitanjali Yadav Rajesh S. Gokhale Debasisa Mohanty

NRPS-PKS is web-based software for analysing large multi-enzymatic, multi-domain megasynthases that are involved in the biosynthesis of pharmaceutically important natural products such as cyclosporin, rifamycin and erythromycin. NRPS-PKS has been developed based on a comprehensive analysis of the sequence and structural features of several experimentally characterized biosynthetic gene clusters...

Journal: :Experimental neurology 2002
Qi-Lin Cao Russell M Howard Jessica B Dennison Scott R Whittemore

Differentiation of pluripotent neural stem cells engrafted into the adult normal and injured spinal cord is restricted to the glial lineage, suggesting that in vitro induction toward a neuronal lineage prior to transplantation and/or modification of the host environment may be necessary to initiate and increase the differentiation of neurons. In the present study, we investigated the differenti...

2008
Nuno Bandeira Julio Ng Dario Meluzzi Roger G. Linington Pieter C. Dorrestein Pavel A. Pevzner

While nonribosomal peptides (NRPs) are of tremendous pharmacological importance, there is currently no technology capable of highthroughput sequencing of NRPs. Difficulties in sequencing NRPs slow down the progress in elucidating the non-ribosomal genetic code and negatively affect various screening programs aimed at the discovery of natural compounds of medical importance. We propose to employ...

2012
Sheila Marie Pimentel-Elardo Lubomir Grozdanov Sebastian Proksch Ute Hentschel

Genomic mining revealed one major nonribosomal peptide synthetase (NRPS) phylogenetic cluster in 12 marine sponge species, one ascidian, an actinobacterial isolate and seawater. Phylogenetic analysis predicts its taxonomic affiliation to the actinomycetes and hydroxy-phenyl-glycine as a likely substrate. Additionally, a phylogenetically distinct NRPS gene cluster was discovered in the microbial...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید