Toxicologic data on cadmium (Cd) indicate that intracellular metallothionein (MT) is protective for Cd exposure, whereas extracellular Cd-containing MT might be toxic. Moreover, Cd is suspected to be a carcinogen though the underlying mechanism is not known. Here we report on the genotoxic activity of cadmium/zinc-metallothionein (Cd/Zn-MT) in a cell-free test system: a concentration-dependent ...

Alkaline phosphatase of Escherichia coli (a dimeric zinc metalloenzyme of identical subunits) containing either 2 catalytic Zn(II) ions or 2 catalytic Cd(I1) ions shows absolute negative cooperativity of phosphate binding as determined by 31P NMR of enzyme-bound phosphate species. With excess phosphate at millimolar concentrations, only 1 mol of noncovalent phosphate complex, E.P (31P resonance...

The present study aims to identify the impact of polluted aquatic body i.e. River Hindon on two selected riparian flora i.e. Azadirachta indica and Acacia nilotica. During the course of study the average concentration of different metals in river water was found as Iron (Fe) 11.27ppm±3.50, Manganese (Mn) 4.00ppm±1.26, Cadmium (Cd) 0.08ppm±0.07, Nickel (Ni) 0.63ppm±0.17 and Zinc (Zn) 1.46ppm±0.3...

levels of the heavy metals copper (cu), zinc (zn), lead (pb), cadmium (cd), chromium (cr), nickel (ni), iron (fe) and manganese (mn) were determined by graphite furnace atomic absorption spectrophotometer (model 67og) in coastal water, sediments and soft tissues of the saccostrea cucullata, from the intertidal zone at five stations in the gulf of chabahar on the iranian coasts along the oman se...

Although the activating effect of metal ions, such as Co++ and Mn*, on a variety of aminopeptidases has long been known (see Smith (2)), no requirements of the pancreatic proteinases for these ions have been demonstrated. It has been found, however, that calcium salts improve the yield of trypsin from trypsinogen (3) and that they increase the stability of trypsin at alkaline pH (4, 5). Earlier...

The isoforms of rat liver apo-metallothionein (MT) were reconstituted in vitro with Cd and Zn ions to study the order of binding of the seven metal sites. Reconstitution with seven Cd ions resulted in a metalloprotein similar to induced Cd,Zn-MT by the criteria of electrophoretic mobility, insensitivity to proteolysis by subtilisin and the pH-dependent release of Cd. Proteolytic digestion of MT...

The incidence of rheumatoid arthritis (RA) has been increased among people who possess habit of tobacco smoking. In the present study, zinc (Zn), copper (Cu), manganese (Mn), lead (Pb) and cadmium (Cd) were determined in scalp hair samples of smokers and nonsmokers RA patients, residents of Dublin, Ireland. For comparison purposes scalp hair samples of age and sex matched healthy smokers and no...

The present study the selected heavy metals such as cadmium (Cd), chromium (Cr), lead (Pb), Manganese (Mn) and cooper (Cu) were determined in water, sediment and gill, liver, muscle of fish H. fossilis, the concentration of Cd, Cr, Pb, Cu Mn and Zn was observed 0.009, 0.056, 0.056, 0.058, 0.049, 0.883, 0.36 mg/l in river water and 3.38, 20.11, 81.53, 258.48, 258.48, 3.40 μg/g in sediment respec...

A new chelating ligand [4-methyl-2,6-bis-(pyridin-2-yl-hydrazonomethyl)-phenol] (1) was prepared by the condensation of 2-hydrazinylpyridine with 2,6-diformyl-p-cresol. Compound 1 exhibits weak fluorescence due to intramolecular photoinduced electron transfer (PET). The sensor (1) demonstrates Zn(2+)-specific emission enhancement due to the “PET off” process through a 1:1 binding mode with the ...