The chloroplast-associated form of superoxide dismutase from maize (Zea mays L.) (SOD-1) has been purified by a stepwise procedure consisting of (NH(4))(2)SO(4) fractionation, G-100 Sephadex gel filtration, DEAE-Sephacel chromatography, and hydroxylapatite chromatography. This procedure resulted in a single band on sodium dodecyl sulfate-polyacrylamide gels indicating that the preparation is ho...

Distribution and availability of heavy metals to plants is important when assessing the environmental quality of an area. The objectives of this study conducted in 2002 were: a) determine the levels of the heavy metals cadmium (Cd), copper (Cu), lead (Pb), manganese (Mn), nickel (Ni) and zinc (Zn) in the agricultural soils of the Bursa plain so that the degree of pollution could be ascertained,...

A chromatographic procedure for purification of the proteins in human parotid saliva has been developed. The eluates of a Sephadex G-150 and two ion exchange columns have been analyzed simultaneously by several physical and chemical tests; these include three optical properties of proteins, assays for neutral sugars, sialic acid and zinc, and disc gel electrophoresis. The ratios of the differen...

The Saccharomyces cerevisiae PEP3 gene was cloned from a wild-type genomic library by complementation of the carboxypeptidase Y deficiency in a pep3-12 strain. Subclone complementation results localized the PEP3 gene to a 3.8-kb DNA fragment. The DNA sequence of the fragment was determined; a 2,754-bp open reading frame predicts that the PEP3 gene product is a hydrophilic, 107-kDa protein that ...

The Isua Supracrustal Belt, Greenland, of Early Archean age (3.81-3.70 Ga) represents the oldest crustal segment on Earth. Its complex lithology comprises an ophiolite-like unit and volcanic rocks reminiscent of boninites, which tie Isua supracrustals to an island arc environment. We here present zinc (Zn) isotope compositions measured on serpentinites and other rocks from the Isua supracrustal...

conclusions as a result of the full range uv-vis scanning of the responsible band in the hptlc experiments (200-700 nm), the flavonoid was the most imaginable natural compound. results through the bioassay guided fractionation method, the greatest antibacterial activities were related to the n-hexane extract. it was also revealed that the effective anti-mrsa agent of the assessed plant was a re...

Protein import into the peroxisome matrix is mediated by peroxisome-targeting signals (PTSs). We have developed a novel, quantitative, in vitro assay for measuring peroxisomal import of PTS1-containing proteins. This enzyme-linked immunosorbent assay-based system utilizes semi-intact human A431 cells or fibroblasts and a biotinylated version of the PTS1-containing import substrate, luciferase. ...

Several methods have been used in the investigation of trace elements in eye tissues. BurdonCooper (1928) and Burdon-Cooper & Lewis (1929) give the results of emission spectrographic analysis of the mineral constituents of normal and cataractous human-eye lenses. The technique failed to demonstrate the presence of zinc, iron and manganese, which Tauber & Krause (1943) were later able to detect ...

introduction: application of organic fertilizers in agricultural soils with low organic matter content is one of the best ways of nutrientsaddition to these soils. different organic fertilizers have different effects on nutrient availability in soil. moreover study of the distribution of nutrients in the soil allows investigating their mobility and bioavailability. the nutrients availability an...