RNA polymerases undergo substantial structural and functional changes in transitioning from sequence-specific initial transcription to stable and relatively sequence-independent elongation. Initially, transcribing complexes are characteristically unstable, yielding short abortive products on the path to elongation. However, protein mutations have been isolated in RNA polymerases that dramatical...

Cultures of the CRL-1606 hybridoma (ATCC) have been reported to undergo continuous proliferation with simultaneous death during nutrient limited fed-batch fermentations. The bcl-2 proto-oncogene has been shown to prevent cell death under a variety of otherwise death inducing conditions. We were interested in elucidating the nature of the massive death observed in cultures of CRL-1606, specifica...

The phenomenon of abortive infection in the Micrococcus lysodeikticus-bacteriophage system described by Naylor and Burgi (1956) constituted the basic problem to which the following study was directed. The role of deoxypentose nucleic acids (DNA) in cellular physiology and the role of certain of them in transformation experiments is well known. (Avery et al., 1944; Hotchkiss, 1951; Zamenhof et a...