نتایج جستجو برای: wide hybridization
تعداد نتایج: 413034 فیلتر نتایج به سال:
We report the specific transduction, via surface stress changes, of DNA hybridization and receptor-ligand binding into a direct nanomechanical response of microfabricated cantilevers. Cantilevers in an array were functionalized with a selection of biomolecules. The differential deflection of the cantilevers was found to provide a true molecular recognition signal despite large nonspecific respo...
Generation of nanomechanical cantilever motion from biomolecular interactions can have wide applications, ranging from high-throughput biomolecular detection to bioactuation. Although it has been suggested that such motion is caused by changes in surface stress of a cantilever beam, the origin of the surface-stress change has so far not been elucidated. By using DNA hybridization experiments, w...
Conducting polymers (CPs) are a group of polymeric materials that have attracted considerable attention because of their unique electronic, chemical, and biochemical properties. This is reflected in their use in a wide range of potential applications, including light-emitting diodes, anti-static coating, electrochromic materials, solar cells, chemical sensors, biosensors, and drug-release syste...
in this study, we explained a nanobiosensor for dna sequence detection, featuring sequence specificity, cost efficiency, speed, and ease of use. without the need for labels or indicators, it may be ideal for the detection of biological agents. this review describes recent advances in electrochemical impedance spectroscopy (eis) with an emphasis on using nanoparticles and nanotechnology. a power...
many researchers consider the use of different probes for hybridization assays as suitable for studying the genetic diversity of nitrogen fixing bacteria. in this study for asessing genetic diversity among bradyrhizobium japonicum isolates, two different probes (suca and topa) chosen from the chromosomal genome of bradyrhizobium strain usda 110 were designed, evaluated by dnaman software and im...
The presence of the vbrA gene encoding the transcriptional antiterminator NusG equivalent protein of Streptomyces virginiae was tested for in 73 Streptomyces species by Southern hybridization. Fifty-five strains (75%) including S. griseus, S. lividans TK-21 and S. coelicolor A3(2) showed clear hybridization signals, indicating wide distribution of vbrA or vbrA homologs in Streptomyces species. ...
We describe here a simple and versatile method of fluorescence in situ hybridization (FISH) on paraffin-embedded tissue sections with specific application in the study of microchimerism, that is, the presence of intact foreign cells within an individual. This is accomplished through the use of X and Y chromosome-specific probes to identify the presence of male nuclei within a tissue section fro...
دورگه سازی در محل، روشی است که در آن از پروب های اسید نوکلئیک برای بررسی انواع تغییرات ژنتیکی در سلول های دست نخورده و بافت های تثبیت شده استفاده می شود. مراحل مختلف این روش شامل انتخاب پروب، آماده سازی نمونه، تیمار پیش از دورگه سازی، دورگه سازی، شستشو، آشکارسازی و روند کنترل می باشند. انتخاب پروب مناسب یکی از جنبه های مهم انجام فرایند دورگه سازی موفقیت آمیز است. حساسیت و ویژگی in situ hybridiz...
Hybridization is an important method to widen variations and develop novel varieties in plants. The increasing interest Stevia rebaudiana over the last decade soared by its potential as alternative source of sugar. In this study, improvement stevia has been conducted through hybridization using Trigona for development hybrids. Trigona, which consists small medium-sized bees, usually found tropi...
In recent years, a wide range of contributions have sought to conceptualize the emergent effects platforms on contemporary capitalism(s). One strand literature has emphasized novelty platforms, stressing their disruptive features and proclaiming rise new era – platform/digital capitalism. Another tended position within longue durée capitalist transformation, focusing continuities historical rec...
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