abstract in october 2009, in one of the alstroemeria (alatroemeria sp.) greenhouses, located in khorasan razavi province, tospovirus-like symptoms (necrosis) were observed on stems, petioles, flowers and leaves of some plants. sap extracts from these plants were mechanically inoculated to indicator plants. necrotic local lesions were observed on petunia hybrida and chenopodium quinoa. necrotic ...

White spot syndrome virus (WSSV) is a major pathogen of penaeid shrimp. Here we identified a new WSSV strain, WSSV-CN04, from naturally infected Marsupenaeus japonicus. Whole genomic sequencing results indicate that the WSSV-CN04 genome was 281 054 bp in length, and encoded 157 hypothetic proteins. The genome sequence of WSSV-CN04 was most closely related to the low-virulent strain WSSV-CN03, s...

Certain parasites can cause severe diseases in shrimp. Among these, protozoa are very important. The objective of our study was to describe the signs, prevalence, and intensity of hemocytosis, a new disease in shrimp, Fenneropenaeus indicus. The experimental design included three groups, one treatment as infected shrimp or group B and 2 controls with non-infected but exposure to White Spot Synd...

Certain parasites can cause severe diseases in shrimp. Among these, protozoa are very important. The objective of our study was to describe the signs, prevalence, and intensity of hemocytosis, a new disease in shrimp, Fenneropenaeus indicus. The experimental design included three groups, one treatment as infected shrimp or group B and 2 controls with non-infected but exposure to White Spot Synd...

The resistance of white spot lesions to acid demineralization when etched with phosphoric acid and sealed with a resin material was studied. Artificially created white spot lesions were sealed and re-exposed to an artificial caries medium along with unprotected white spot lesions. Examination with polarized light microscopy revealed continued progression of the unprotected white spot lesions, w...

White spot disease (WSD) is caused by the white spot syndrome virus (WSSV), which results in devastating losses to the shrimp farming industry around the world. However, the mechanism of virus entry and spread into the shrimp cells is unknown. A binding assay in vitro demonstrated VP28-EGFP (envelope protein VP28 fused with enhanced green fluorescence protein) binding to shrimp cells. This prov...

Although aquaculture in Ecuador has a high economic and socio-cultural importance, pathogenic microorganisms affect the development vitality of crustaceans, fish, mollusks, reducing their production yields. Among these pathogens, White Spot Syndrome Virus (WSSV) is an invertebrate virus that induces mortality, generating severe losses due to its wide geographical distribution infection rate fin...

White spot syndrome virus (WSSV) causes large economic losses to the shrimp aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. In this study, we present the development of a novel real time isothermal recombinase polymerase amplification (RPA) assay for WSSV detection on a small ESEQuant Tube Scanner device. The RPA sensitivity,...