It has been reported [Smith, McWilliams & Hass (1986) Biochem. Biophys. Res. Commun. 136, 336-340] that addition of certain metal ions, notably Co2+ and Mn2+, promoted the refolding of denatured phosphoglycerate mutase from wheat germ. We have re-investigated these experiments and have shown that, when precautions are taken to avoid artefacts in the assay system, the metal ions do not promote a...

Rational: Smoking kills more than one billion current smokers worldwide prematurely due to various tobacco-related diseases. Smoking contributes to multiple well-documented adverse health effects, including heart disease, pulmonary disease and lung and other cancers in both industrialized and developing countries. Tobacco smoking is a leading cause of preventable disease and death. If the curre...

ABSTRACT Host plant resistance is an economical and environmentally sound method of control of leaf rust caused by the fungus Puccinia triticina, which is one of the most serious diseases of wheat (Triticum aestivum) worldwide. Wild relatives of wheat, including the tetraploid T. timopheevii subsp. armeniacum, represent an important source of genes for resistance to leaf rust. The objectives of...

An amino acid-incorporating system has been obtained from ungerminated wheat germ, which can be stimulated by the addition of uridylic-rich polynucleotides. Polyadenylic and polycytidylic acids fail to stimulate the incorporation of lysine and proline, respectively. The amino acid codons determined by the use of the uridylic-rich polynucleotides coincide almost perfectly with those reported for...

We used thin-section fracture-label to determine the distribution of wheat-germ agglutinin binding sites in intracellular membranes of secretory and nonsecretory rat tissues as well as in human leukocytes. In all cases, analysis of the distribution of wheat germ agglutinin led to the definition of two endomembrane compartments: one, characterized by absence of the label, includes the membranes ...

This study examined the supercritical fluid extraction of wheat germ oil. The effects of pressure (200-300 bar at 40 degrees C) and extraction time on the oil quality/quantity were studied. A comparison was also made between the relative qualities of material obtained by SFE and by organic solvent extraction. The extracts were analyzed for alpha-tocopherol and polyunsaturated fatty acid content...

Uridine diphosphate (UDP)-glucose 4-epimerase (EC 5.1.3.2) has been purified over 1000-fold from extracts of wheat germ by MnCl(2) treatment, (NH(4))(2)SO(4) fractionation, Sephadex column chromatography, and adsorption onto and elution from calcium phosphate gel. The enzyme has a pH optimum of 9.0. Km values are 0.1 mm for UDP-d-galactose and 0.2 mm for UDP-d-glucose. NAD is required for activ...

The capacity of polyadenylated RNA from developing castor bean endosperm to program protein synthesis in a wheat germ cell-free translational system has been examined. Although the use of micrococcal nuclease-treated wheat germ extracts demonstrated a low but significant content of translatable mRNA in dry seeds, a large scale increase in total translational capacity was observed during germina...