IN the analysis of events immediately preceding and following fertilization of the frog egg, it is frequently desirable to treat the eggs with various reagents. Any such treatment must circumvent the barrier represented by the fertilization membrane and the jelly layers. These membranes can be removed from fertilized eggs mechanically or by treatment with proteolytic enzymes (Spiegel, 1951) wit...

Background: Chronic diabetes mellitus is accompanied with enhanced oxidative stress and reduce the activity of antioxidant defense system. Due to significant role of enhanced oxidative stress in development of testes damage in diabetices, this study was conducted to evaluate the effect of royal jelly on oxidative stress marker in testis tissue of diabetic rats. Materials and Methods: In this ex...

Objective(s):Paclitaxel is a potent chemotherapy agent with severe side effects, including allergic reactions, cardiovascular problems, complete hair loss, joint and muscle pain, which may limit its use and lower its efficiency. The cardioprotective effect of royal jelly was investigated on paclitaxel-induced damages. Materials and Methods:Adult male Wistar rats were divided into control and te...

Objectives: The aim of the present study was to evaluate protective effect of royal jelly on sperm parameters, testosterone level, and malondialdehyde (MDA) production in mice. Materials and Methods: Thirty-two adult male NMRI mice weighing 30±2 g were used. All the animals were divided into 4 groups. Control group: received saline 0.1 ml/mouse/day orally for 30 days. Royal Jelly group (RJ): re...

Distant sequence relationships in proteins containing the beta jelly-roll fold were investigated using sensitive sequence comparison methods, including PSI-BLAST and Hidden Markov Models. A relationship was identified between the rmlC-like and phosphomannose isomerase SCOP (version 1.53) superfamilies, which were merged in the most recent SCOP release. No other distant sequence relationships li...

Several well-known morphogenetic gradients and cellular movements occur along the dorsal/ventral axis of the Drosophila embryo. However, the current techniques used to view such processes are somewhat limited. The following protocol describes a new technique for mounting fixed and labeled Drosophila embryos for coronal viewing with confocal imaging. This method consists of embedding embryos bet...

Particulate matter export fuels benthic ecosystems in continental margins and the deep sea, removing carbon from the upper ocean. Gelatinous zooplankton biomass provides a fast carbon vector that has been poorly studied. Observational data of a large-scale benthic trawling survey from 1994 to 2005 provided a unique opportunity to quantify jelly-carbon along an entire continental margin in the M...

The extracellular matrix components show specific distribution patterns within various structures of the umbilical cord, among which Wharton's jelly is especially collagen-rich tissue. Cathepsin L is a potent cysteine protease engaged in degradation of extracellular matrix proteins, including collagens. We evaluated the activity and expression of cathepsin L, and the inhibitory effect of cystei...

OBJECTIVES This study aims to investigate the effects of radiation on serum trace elements and the changes in these elements as induced by royal jelly in rats undergoing head and neck irradiation. MATERIALS AND METHODS Thirty-two Sprague-Dawley male rats at the age of eight weeks with a mean weight of 275±35 g were included in the study. Subjects were divided into four groups with eight rats ...