Foodborne Enteropathogenic Escherichia coli (EPEC) infections of the small intestine cause diarrhea especially in children and are a major cause of childhood death in developing countries. EPEC infects the apical membrane of the epithelium of the small intestine by attaching, effacing the microvilli under the bacteria and then forming microcolonies on the cell surface. We first asked the questi...

prosite database contains a set of entries corresponding to protein families, which are used to identify the family of a protein from its sequence. although patterns and profiles are developed to be very selective, each may have false positive or negative hits. considering false positives as items that reduce the selectiveness of a pattern, then, the more selective pattern we have, a more accur...

recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...

Objective: To develop a lentiviral vector system, which can be used to co-express multiple genes of interest, such as siRNAs cassettes, reporter gene and resistant gene simultaneously and to facilitate to titrate lentiviral vector stock with TCID50 method. Method: Synthesis new multiple cloning sites(MCS)to replace the original MCS of pLKO-1-puro and produced an intermediate plasmid pLKO-1-puro...

background: in vitro simulation of developmental processes is an invaluable tool to shed light on the intrinsic mechanism of developmental biosystems such as central nervous system in mammals. chick somites have been used to simulate the neural differentiation of human neural progenitor cells. in the present study, we aimed to indicate whether somites have the ability to express required neural...

a sweet water-soluble protein that reminds stable over wide ranges of temperature and ph, brazzein has various applications. its tastes like cane sugar but have no calories. however, the extraction of brazzein from its natural source is expensive and not applicable. in this study we used recombinant dna technology to provide an alternative option for cheaper mass production of brazzein. a brazz...

we present the first reported case of antiphospholipid syndrome with stroke in an iranian boy (7-month-old) who had two ischemic strokes within a period of 2 months. serum anticardiolipid antibody was positive and the patient had low levels of protein s and c. this case emphasizes the importance of antiphospholipid antibody in children with unexplained ischemic stroke.

animal agricultural production systems are major sources of nonpoint pollution affecting quality of water sources. nitrogen has been identified as the foremost source of nonpoint water pollution  and the potential negative impacts of n have become an area of public concern. protein degradation from feed ingredients is an important factorinfluencing aa supply to the duodenum. ruminal proteolysis...