the success rate of embryo production critically depends on several factors. one of the pivotal factors is a technique used for the whole procedure of in vitro fertilization (ivf) and in vitro embryo culture (ivc) (1, 2). besides the technique selected for ivf / in vitro embryo production (ivep), there is an important consideration called ivf/ivep culture dishes disinfection (3). for many years...

the aim of the present study was to develop an in vitro system for functional investigation of ovarian follicular cells in sterlet, acipenser ruthenus. oocytes for the primary culture were obtained from the ovaries of a 6 years old sterlet 729 g in weight and 47 cm in total length. the oocytes were in advanced vitellogenesis stage (pi >10). a part of the ovary (containing about 300 follicles) w...

the aim of this study was to establish and optimise a simplified method of halved grain culture, in order to utilise it to evaluate the ability of isolated sinks (developing wheat grains) in sucrose uptake and conversion (defined by sink strength) under unlimited source conditions. both sucrose uptake and starch synthesis increased linearly over the incubation periods used in this study. a ...

this study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (ivf) of goat oocytes. cumulus oocytes complexes (cocs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. upon grading, only normal quality cocs were maturated in tcm-199 for 48 hours. the percentage of cocs reached to the m-ii sta...

scented rose species are very important in the genus rosa due to their use in highly-prized essential oil production and as garden roses. the fragrance of the rose flower (attar) captured by extraction is one of the most valuable flavor and fragrance products. a comprehensive study of the papers published in the field of in vitro culture and biotechnology of these species, particularly damask r...

background and purpose: candida albicans is the most common causative agent of candidiasis. candidiasis management is dependent on the immune status of the host, severity of disease, and the choice of antifungal drug. antifungals, specifically triazoles, are widely administered for the treatment of invasive fungal infections. herein, we aimed to evaluate the in vitro susceptibility of c. albica...

background: luteal phase support is mandatory in art (assisted reproductive technologies) for optimizing outcome, so the luteal phase is supported with either progesterone, addition of estradiol to progesterone, hcg or gonadotropin releasing hormone (gnrh) agonists. supplementation of luteal phase with progesterone is prescribed for women undergoing routine ivf treatment. objective: to compare ...