Cryopreservation of matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies after warming. Even though rats, like mice, have been used in various research fields including reproductive technology, information about cryopreservation of rat oocytes is limited. The objective of the present study was to improve the vitrification protocol for...

background: retinoids have been suggested to play a role in oogenesis and oocyte survival. objective: in the present study the effects of retinol palmitate were investigated on differential follicular counts in response to superovulation as well as follicle quality after vitrification of ovaries. materials and methods: ten, 4 week old female balb/c mice were randomly assigned to either paraffin...

STUDY QUESTION Does the human embryo growth rate affect the outcome of vitrified-warmed blastocyst transfer? SUMMARY ANSWER Following vitrification, the incidence of abnormal spindle morphology was increased and the implantation competence was decreased in growth-retarded embryos compared with normally developing embryos. WHAT IS KNOWN ALREADY Various types of spindle abnormality occur in h...

UNLABELLED OBJECTIVES This study sought to evaluate the outcome of fresh and vitrified-warmed cleavage-stage and blastocyst-stage embryo transfers in patients undergoing ART treatment within an ethnic Chinese population. STUDY DESIGN We compared the clinical results of embryo transfer on the 3rd (cleavage stage) or 5th (blastocyst stage) day after oocyte retrieval, including clinical pregn...

BACKGROUND A successful oocyte vitrification program is important for women with various indications for assisted reproduction technology. The objective of this study was to report the outcome of vitrification of oocytes, obtained through an oocyte donation program, by evaluating the embryo development, pregnancy and implantation rates (IRs) after blastocyst transfer. METHODS A total of 1098 oo...

The effectiveness of different cryodevices (open-pulled straw (OPS), electron microscopy grid (EMG), and Cryotop was evaluated for vitrification of immature bovine oocytes. Polar body, metaphase II stage (MII), survivability, and subsequent developmental rates were determined. Only oocytes with four or five layers of cumulus cells were used. Oocytes were equilibrated in two vitrification soluti...

Background: Ovarian cryopreservation is an efficient way to preserve fertility of cancer patients before chemotherapy. This study was designed to evaluate the effect of apha lipoic acid (ALA) on reactive oxygen species (ROS) production and total antioxidant capacity (TAC) of cultured isolated pre-antral follicles derived from vitrified and non-vitrified ovarian tissue. Materials and Methods: Im...

background: embryo cryopreservation is the process that water is removed from the cell by cryoprotectant materials, and embryos are stored at temperature below zero. this process may affect the viability and developmental potential of embryos. objective: in this study, the effect of the vitrification cryotop method on the expression level of oct4 and mest developmental genes in mouse blastocyst...

Background: The development of a reliable method for the cryopreservation of mammalian ovary would be an important advancement in the field of reproductive biology for the preservation of genetic resources. The cryopreserved follicles have the potential to develop in-vitro; however, the developmental rate is lower than that of fresh follicles. Researchers have used that of different cryoprotect...

s:2022:"Background: Cryopreservation is an important option for preserving the fertility. We examined the reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and developmental competence, of vitrified isolated mice pre-antral follicles as an alternative method for restoring fertility. Materials and Methods: The 140-160 μm pre-antral follicles mechanically isolated from ov...