Two alleles for viomycin-capreomycin resistance (vic) in Mycobacterium smegmatis affect ribosome structures. One (vicA) affects a component of 50S subunits and the other (vicB) affects a component of 30S subunits. The locus for neomycin-kanamycin resistance (nek), which is linked to vicA and vicB, affects a component of 30S subunits. Although the erythromycin resistance locus (ery) is linked to...

To elucidate the physiological roles of endothelin-1 (ET-1) and endothelin-2 (ET-2)/vasoactive intestinal contractor (VIC) in gastric injury of mice, we measured the gene expression rates of ET-1 and ET-2/VIC in gastric injury induced by ethanol in young (8 weeks) and old (>33 weeks) mice. Mice that were fasted for 24 h were injected with absolute ethanol intragastrically and killed after 1 or ...

Biophysical cues are widely recognized to influence cell phenotype. While this evidence was established using static substrates, there is growing interest in creating stimulus-responsive biomaterials that better recapitulate the dynamic extracellular matrix. Here, a clickable, photodegradable hydrogel substrate that allows the user to precisely control substrate elasticity and topography in sit...

I. E. Khodasevych, I. V. Shadrivov, D. A. Powell, W. S. T. Rowe, and A. Mitchell School of Electrical and Computer Engineering, RMIT University, Melbourne, VIC 3001, Australia ARC Centre for Ultrahigh bandwidth Devices for Optical Systems (CUDOS), Melbourne, VIC 3001, Australia Nonlinear Physics Centre, Research School of Physics and Engineering, Australian National University, Canberra, ACT 02...

This study documents that chlorinated analogs of leukotoxin diol 1, in which the vic-diol has been replaced with vic-chlorides (2), induce caspase 3 activity and apoptosis on HepG2 cells in a dose-dependent manner in analogy to the parent diol. This suggests that chlorides may substitute for hydroxyls in certain lipids as bioisosteres in defined biological settings.

Main Article: Reflection on the Development of a Research Agenda in Rural Health Lisa Bourke Department of Rural Health, The University of Melbourne PO Box 6500, Shepparton, VIC 3632, AUSTRALIA [email protected] James D. Best Lee Kong Chian School of Medicine Nanyang Technological University, Novena Campus 11 Mandalay Road, SINGAPORE 308232 [email protected] John Wakerman Faculty of Medi...

The regulation of phosphate (Pi) handling is crucial during calcification of the aortic valve. Gene profiling of Pi transporters revealed that VIC culture expresses SLC201A1/Pit1 and SLC20A2/Pit2. On exposure to a mineralizing medium (2 mM Pi), the expression of Pi transporters in VIC culture is increased several folds, with the highest magnitude for SLC20A1. By using siRNAs, we established tha...

Dustin J. Flanagan,1,2,10 Toby J. Phesse,3,10,11 Nick Barker,4,5,10 Renate H.M. Schwab,1,2 Nancy Amin,1,2 JordaneMalaterre,6 Daniel E. Stange,7 Cameron J. Nowell,3 Scott A. Currie,1,2 Jarel T.S. Saw,1,2 Eva Beuchert,1,2 Robert G. Ramsay,6 Owen J. Sansom,8 Matthias Ernst,3,11 Hans Clevers,7 and Elizabeth Vincan1,2,9,* 1Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, V...

Heart valve disease is an important cause of morbidity and mortality worldwide. Little is known about valve disease pathogenesis, but increasing evidence implicates a genetic basis for valve disease, suggesting a developmental origin. Although the cellular and molecular processes involved in early valvulogenesis have been well described, less is known about the regulation of valve extracellular...