Necrotising fasciitis is a rapidly progressing necrotising process involving subcutaneous tissue and fascia but sparing muscles, and is accompanied by severe systemic toxicity.1 2The syndrome is often caused by group A streptococci,' 3 but it is now recognised that other bacteria may be involved, usually in mixed culture. Organisms that have been implicated include a variety of anaerobes, enter...

Vibrio vulnificus secrets a pore-forming toxin called Vibrio vulnificus hemolysin (VVH). In this study, we showed that methyl-beta-cyclodextrin (MβCD), an oligosaccharide, decreased binding of VVH to Chinese hamster ovary (CHO) cells, resulting in inhibition of its cytotoxicity. When the VVH was incubated with MβCD, cytotoxicity of the toxin was inhibited from 100.3 ± 7.2% to 19.6 ± 5.3%. Bindi...

vulnificus biotype 2 Eva Sanjuán, Fernando González-Candelas , and Carmen Amaro. Departamento de Microbiología, Facultad de Biología, Universidad de Valencia, Valencia, España. ([email protected], [email protected]) 2 Unidad mixta “Genómica y Salud” Centro Superior de Investigación en Salud Pública-Universidad de Valencia/Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Valencia, Es...

We demonstrated that trifluoperazine, a calcium-calmodulin antagonist, blocked the hyperpermeability induced by Vibrio vulnificus cytolysin in in vitro-modeled endothelium and prevented the deaths of mice. Furthermore, compared to tetracycline alone, tetracycline combined with trifluoperazine enhanced the survival rate of V. vulnificus-infected mice, indicating the role of the cytolysin as an i...

Vibrio vulnificus is a Gram-negative bacterium which is associated with severe infections in humans. We experienced two cases of sucrose-fermenting V. vulnificus infection. The causative agents in both cases were unidentifiable by conventional identification systems because of their unique characteristics, and sequencing of 16S rDNA was found to be useful for diagnosis.

The present work describes a retrospective study aiming to verify a possible correlation between the environmental conditions (temperature, salinity and dissolved oxygen), the abundance of Vibrio spp., and the prevalence of V. parahaemolyticus and V. vulnificus in the Manila clam R. philippinarum harvested in Sacca di Goro, Emilia-Romagna Region, Northern Italy. On the whole, 104 samples, colle...

The effect that climate change and variability will have on waterborne bacteria is a topic of increasing concern for coastal ecosystems, including the Chesapeake Bay. Surface water temperature trends in the Bay indicate a warming pattern of roughly 0.3-0.4°C per decade over the past 30 years. It is unclear what impact future warming will have on pathogens currently found in the Bay, including V...

The in vitro effects of the Perkinsus marinus serine protease on the intracellular survival of Vibrio vulnificus in oyster hemocytes were examined by using a time-course gentamicin internalization assay. Results showed that protease-treated hemocytes were initially slower to internalize V. vulnificus than untreated hemocytes. After 1 h, the elimination of V. vulnificus by treated hemocytes was ...

A dual function blue fluorescent protein from Vibrio vulnificus is also an NADPH-dependent oxidoreductase, rendering it a useful tool for biophysical studies.

Vibrio vulnificus is a naturally occurring bacterium found ubiquitously in coastal waters. Infection with this organism, which is often associated with eating raw oysters, is the leading cause of seafood-related deaths in the United States. This article reviews the current scientific literature on this potentially lethal pathogen and discusses the prognosis, treatment, and prevention of V. vuln...