نتایج جستجو برای: vaca protein

تعداد نتایج: 1235901  

2016
Nora J. Foegeding Rhonda R. Caston Mark S. McClain Melanie D. Ohi Timothy L. Cover

The VacA toxin secreted by Helicobacter pylori enhances the ability of the bacteria to colonize the stomach and contributes to the pathogenesis of gastric adenocarcinoma and peptic ulcer disease. The amino acid sequence and structure of VacA are unrelated to corresponding features of other known bacterial toxins. VacA is classified as a pore-forming toxin, and many of its effects on host cells ...

Journal: :Biochemical and biophysical research communications 2015
Shota Nakashima Tomoyuki Kakugawa Hirokazu Yura Masaomi Tomonaga Tatsuhiko Harada Atsuko Hara Shintaro Hara Masayuki Nakano Eiki Yamasaki Noriho Sakamoto Yuji Ishimatsu Hajime Isomoto Bernadette R Gochuico Anthony F Suffredini Hiroshi Mukae Hisao Kurazono Toshiya Hirayama Joel Moss Shigeru Kohno

OBJECTIVE Prior reports suggested that infection with Helicobacter pylori was associated with respiratory diseases; pathogenetic mechanisms however, were not defined. We tested the hypothesis that VacA, an exotoxin of H. pylori, a gastric pathogen, was aspirated into the lung and could stimulate secretion of inflammatory cytokines by lung epithelial cells. METHODS The presence of VacA was det...

Journal: :Journal of medical microbiology 2005
Nicola Fitchen Darren P Letley Paul O'Shea John C Atherton Paul Williams Kim R Hardie

The major secreted virulence factor of Helicobacter pylori, the vacuolating cytotoxin VacA, is known to insert into eukaryotic membranes and has been observed in association with the surface of H. pylori cells that are actively producing it. Here, it is demonstrated that VacA is capable of interacting with the surface of H. pylori and Escherichia coli after secretion. It is shown that this inte...

لطیفی نوید, سعید, جبارپور بنیادی, مرتضی , صفرعلیزاده, رضا , صومی, محمد‌حسین , متقی, بتول , مهدوی, مجید ,

Background: Helicobacter pylori vacA (vacuolating toxin A) gene is comprised of mid- (m), intermediate- (i) and signal-regions. Recently, the vacA-i region genotype has been suggested to be a better predictor of disease severity than either the s- or m-region. The main aim of the present study was to determine the associations of i region poly-morphisms of vacA gene with gastric cancer (GC) and...

Journal: :Gut 1997
K Ogura F Kanai S Maeda H Yoshida M Ogura K H Lan K Hirota T Kawabe Y Shiratori M Omata

BACKGROUND It has been reported that infection with vacuolating cytotoxin positive Helicobacter pylori strains is associated with gastroduodenal disease in Western countries. AIMS To evaluate the prevalence of cytotoxin producing strains among patients with H pylori infection in relation to gastrointestinal diseases in Japan. PATIENTS Ninety seven patients undergoing endoscopy. METHODS A ...

Journal: :Journal of clinical microbiology 2004
Huseyin Saribasak Barik A Salih Yoshio Yamaoka Ersan Sander

The predominant Helicobacter pylori strains circulating among geographic locations differ in regard to genomic structure. The association of the cagA-positive, vacA s1 genotypes with peptic ulcer disease (PUD) and gastric cancer was reported in Western countries but not in East Asian countries. Strains from Western countries predominantly possessed cagA type 2a, vacA s1a or s1b/m1a, or vacA m2a...

Journal: :Infection and immunity 2010
Heather Parker Kenny Chitcholtan Mark B Hampton Jacqueline I Keenan

Helicobacter pylori bacteria colonize the human stomach where they stimulate a persistent inflammatory response. H. pylori is considered noninvasive; however, lipopolysaccharide (LPS)-enriched outer membrane vesicles (OMV), continuously shed from the surface of this bacterium, are observed within gastric epithelial cells. The mechanism of vesicle uptake is poorly understood, and this study was ...

Journal: :Journal of clinical microbiology 1998
Y Yamaoka T Kodama D Y Graham K Kashima

We compared four tests for antibodies to CagA or VacA, HelicoBlot 2. 0, RIDA Blot Helicobacter, CHIRON RIBA H. pylori SIA, and an enzyme-linked immunosorbent assay using recombinant CagA. Immunoblot assays were accurate for determining Helicobacter pylori status but poor for determining CagA or VacA status (accuracy, 66 to 80% for CagA status and 34 to 67% for VacA status). None can be recommen...

Journal: :Infection and immunity 2002
Dirk Bumann Sevil Aksu Meike Wendland Katharina Janek Uschi Zimny-Arndt Nicolas Sabarth Thomas F Meyer Peter R Jungblut

Secreted proteins (the secretome) of the human pathogen Helicobacter pylori may mediate important pathogen-host interactions, but such proteins are technically difficult to analyze. Here, we report on a comprehensive secretome analysis that uses protein-free culture conditions to minimize autolysis, an efficient recovery method for extracellular proteins, and two-dimensional gel electrophoresis...

Journal: :Jornal de pediatria 2010
Jon A Vanderhoof

O artigo de Lins et al.1 é um estudo importante porque ressalta vários fatores-chave da alergia à proteína do leite de vaca. Os pesquisadores identificaram, com base na história, 66 pacientes com reações adversas à proteína do leite de vaca. Essas reações incluíam uma variedade de sintomas cutâneos, gastrointestinais e sistêmicos. Mediram-se os níveis de imunoglobulina E (IgE) sérica total e es...

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