Rabbit anti-hZTL1 antibody was raised against a synthetic peptide corresponding to amino acids 190–211 of hZTL1 (ILSSPSKRGQKGTLIGYSPEGT) and was affinity purified. Human jejunum was obtained from cadaver donors with appropriate ethics committee approval. Frozen sections (7 mm) were fixed for 30 min on ice with 4 % (w/v) paraformaldehyde in PBS. Caco-2 cells, grown for 14 days on polycarbonate f...

Members of the voltage-gated K(+) (K(V)) channel family are suggested to control the resting membrane potential and the repolarization phase of the action potential in urinary bladder smooth muscle (UBSM). Recent studies report that stromatoxin-1, a peptide isolated from tarantulas, selectively inhibits K(V)2.1, K(V)2.2, K(V)4.2, and K(V)2.1/9.3 channels. The objective of this study was to inve...

The functional role of the voltage-gated K(+) (K(V)) channels in human detrusor smooth muscle (DSM) is largely unexplored. Here, we provide molecular, electrophysiological, and functional evidence for the expression of K(V)2.1, K(V)2.2, and the electrically silent K(V)9.3 subunits in human DSM. Stromatoxin-1 (ScTx1), a selective inhibitor of K(V)2.1, K(V)2.2, and K(V)4.2 homotetrameric channels...

For the families ax = by +z +v +w, a, b = 1, . . . , 100, and ax = by + z + v +w, a, b = 1, . . . , 100, of projective algebraic threefolds, we test numerically the conjecture of Manin (in the refined form due to Peyre) about the asymptotics of points of bounded height on Fano varieties.

PURPOSE To investigate whether a longer sagittal view and less movement using a dual sagittal crystal probe (DSCP) for trans rectal ultra sound (TRUS) allow for more accurate online-planning in I-125 permanent implant brachytherapy of the prostate, compared to a single sagittal crystal probe (SSCP). MATERIAL AND METHODS Between March 2008 and March 2010, 50 patients with prostate cancer were ...

Comparative in vitro susceptibility of Proteus species isolated from clinical specimens to ciprofloxacin, tetracycline and honey was evaluated using standard microbiological techniques. Honey was diluted by percent volume per volume concentration of 100%(neat), 80%(v/v), 60%(v/v), 40% (v/v), 20% (v/v) and 10% (v/v) and its antimicrobial activity against Proteus species compared to ciprofloxacin...

Abstract Dense (Hf, Ta, Nb, Ti, V)C- and (Ta, V, W)C-based high-entropy carbides (HEC) were produced by three different sintering techniques: gas pressure sintering/sinter–HIP at 1900 °C 100 bar Ar, vacuum 2250 0.001 as well SPS/FAST 2000 60 MPa pressure. The relative density varied from 97.9 to 100%, with SPS producing 100% dense samples both compositions. Grain size measurements showed that t...

A CMOS voltage reference, which is based on the weighted difference of the gate–source voltages of an NMOST and a PMOST operating in saturation region, is presented. The voltage reference is designed for CMOS low-dropout linear regulators and has been implemented in a standard 0.6m CMOS technology ( 0 9 V at 0 C). The occupied chip area is 0.055 mm. The minimum supply voltage is 1.4 V, and the ...

Porcine heart was observed to express annexins V (CaBP33) and VI in large amounts, and annexins III and IV in much smaller amounts. Annexin V (CaBP33) in porcine heart was examined in detail by immunochemistry. Homogenization and further processing of heart in the presence of EGTA resulted in the recovery of annexin V (CaBP33) in the cytosolic fraction and in an EGTA-resistant, Triton X-100-sol...

Secondary metabolite contents (total phenolic, flavonoid, tocopherol, and tocotrienol) and antioxidant activities of Hashemi rice bran extracts obtained by ultrasound-assisted and traditional solvent (ethanol and 50:50 (v/v) ethanol-water) extraction techniques were compared. Phenolic and, flavonoid compounds were identified using ultra-high performance liquid chromatography and method validati...