نتایج جستجو برای: using rep
تعداد نتایج: 3393050 فیلتر نتایج به سال:
The putative replication initiation protein (Rep) of Coconut foliar decay virus (CFDV) was expressed as a 6x His recombinant protein in E. coli and in recombinant baculovirus. Purified 6x His-Rep protein was demonstrated to possess sequence non-specific RNA- and ssDNA-binding activities as well as magnesium-dependent ATPase/GTPase activity. The yeast two-hybrid system revealed that CFDV Rep cou...
Recent success achieving long-term in vivo gene transfer without a significant immune response by using adeno-associated virus (AAV) vectors (X. Xiao, J. Li, and R. J. Samulski, J. Virol. 70:8098-8108, 1996) has encouraged further development of this vector for human gene therapy. Currently, studies focus on the generation of high-titer vectors by using the two-plasmid helper-vector system in a...
Repetitive sequence-based PCR (rep-PCR) has been recognized as an effective method for bacterial strain typing. Recently, rep-PCR has been commercially adapted to an automated format known as the DiversiLab system to provide a reliable PCR-based typing system for clinical laboratories. We describe the adaptations made to automate rep-PCR and explore the performance and reproducibility of the sy...
As their names imply, parvoviruses of the genus Dependovirus rely for their efficient replication on the concurrent presence of a helpervirus, such as herpesvirus, adenovirus, or papilloma virus. Adeno-associated virus 2 (AAV2) is such an example, which in turn can efficiently inhibit the replication of each helpervirus by distinct mechanisms. In a previous study we have shown that expression o...
Nucleic acid polymers (NAPs) block the release of subviral particles from hepatocytes, a mechanism consistent with their antiviral activity against hepatitis B virus (HBV) in patients. Analysis of immunostimulatory properties of NAPs were conducted with several NAP species: REP 2006, the prototypic degenerate NAP [dN]40, containing TLR9-stimulatory CpG; REP 2055 a clinically active NAP with a s...
We describe a fluorescence assay that can be used to monitor helicase-catalyzed unwinding of duplex DNA continuously in real time. The assay is based on the observation that fluorescence resonance energy transfer (FRET) occurs between donor (fluorescein) and acceptor (hexachlorofluorescein) fluorophores that are in close proximity due to their covalent attachment to the 3' and 5' ends of the co...
pseudomonas syringae pv. syringae (pss) با دارا بودن بیش از 180 میزبان، بعنوان یکی از مهمترین بیمارگرهای گیاهی تلقی شده و سالیانه خسارات قابل توجهی را در سراسر دنیا به محصولات مختلف وارد می کند. به منظور بررسی خصوصیات ژنوتیپی باکتری مذکور، 58 جدایه از میزبان های مختلف شامل هسته داران، غلات، دانه داران، برخی علف های هرز و گیاهان زینتی در استان های فارس، کهگیلویه و بویراحمد، چهارمحال و بختیاری و ...
سابقه و هدف: جنس آزوسپیریلوم شامل ریزوباکتری‌های آزادزی، تثبیت‌کننده‌ی نیتروژن و محرک رشد گیاه هستند که می-توانند رشد و عملکرد بسیاری از محصولات زراعی مهم از جمله برنج و گندم را تحت تأثیر قرار دهند. این مطالعه با هدف جداسازی و شناسایی گونه‌های بومی مختلف آزوسپیریلوم و بررسی تنوع ژنتیکی آن‌ها به عنوان عوامل محرک رشد گیاه انجام شد.مواد و روش‌ها: در این مطالعه نمونه‌های خاک و ریشه‌ی مزارع گن...
tarkhineh is one of the traditional foods in iran and a rich source of probiotic bacteria. the objective of this study was to identify the probiotic bacteria isolated from tarkhineh using 16s rrna gene sequencing and molecular typing with repetitive extragenic palindromic–pcr (rep-pcr). in total, 20 different bacteria were isolated from traditional dairy products and tarkhineh. molecular identi...
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