The effects of Friend erythroleukemia cells on angiogenesis were studied in chick embryo chorioallantoic membrane assay and in human umbilical vein endothelial cells. In chorioallantoic membrane assay, the conditioned medium of Friend cells stimulated in vivo angiogenesis to an extent comparable to that observed with Prostaglandin El, used as positive control. Prostaglandin El added to conditio...

To explore the effect of fluctuating glucose on endothelial cells, human umbilical vein endothelial cells were incubated for 14 days in media containing different glucose concentrations: 5 mmol/l, 20 mmol/l, or a daily alternating 5 or 20 mmol/l glucose. Apoptosis was studied by different methods: viability assay, cell cycle analysis, DNA fragmentation, and morphological analysis. Furthermore, ...

Growth differentiation factor 15 (GDF15), a direct target gene of p53, is a multifunctional member of the TGF-β/BMP superfamily. GDF15 can be induced and is implicated as a key secretory cytokine in response to multiple cellular stimuli. Accumulating evidence indicates that GDF15 is associated with the development and prognosis of diabetes mellitus, while whether GDF15 can be induced by high gl...

OBJECTIVE The objective of this study were to (1) quantify the concentrations of inhibin A, activin A, and follistatin in maternal serum and umbilical vein (inhibin A, activin A) in IUGR due to placental dysfunction pregnancies and control group, (2) determine the concentration differences of these factors in maternal and umbilical vein serum in control and subject group, and (3) examine the re...

This study demonstrates a mass production method using nanoporous Anodized Aluminum Oxide (AAO) templates as mould insert tools that are used to structure large area polymer surfaces by a micro injection moulding process. SEM and water contact angle measurements served to evaluate the effect of nanostructures on surface properties. Human umbilical vein endothelial cells were cultured on nano-st...

Background: Postpartum haemorrhage is a single major and leading cause of maternal morbidity mortality. PPH the loss more than 500 ml blood following vaginal delivery or 1000 caesarean section. India has mortality ratio 167 per 1 lakh live births. The most common which accounts for 25-30% significant cause. Methods: 200 patients were included in this prospective observational study divided into...

OBJECTIVE To determine whether insulin reverses gestational diabetes mellitus (GDM)-reduced expression and activity of human equilibrative nucleoside transporters 1 (hENT1) in human umbilical vein endothelium cells (HUVECs). RESEARCH DESIGN AND METHODS Primary cultured HUVECs from full-term normal (n = 44) and diet-treated GDM (n = 44) pregnancies were used. Insulin effect was assayed on hENT...

Methods A 35-year-old G3P1 patient, referred to our division of Maternal Fetal Medicine at 29 weeks of gestation due to fetal growth retardation. On the first examination, she was afebrile (36. 6’ C), and had normal blood pressure (100/70 mmHg). Past medical or family history revealed no significance and she had no drug, alcohol or tobacco use. Mild cardiomegaly, splenomegaly and dilatation of ...