background: cobalamin deficiency and peripheral neuropathy (pn) are commonly seen in hiv-infected adults. the level of urine methylmalonic acid (umma), a reliable indicator of tissue cobalamin status, was determined in hiv infected subjects with and without pn to establish this association. methods: one hundred and ninety-eight (198) consenting hiv infected subjects with and without pn were rec...

Introduction. Favipiravir is one of the most well-known broad-spectrum drugs against many RNA viruses, including severe acute respiratory syndrome virus 2 [severe coronavirus (SARS-CoV-2)]. Due to its structure, favipiravir embedded in and blocks further replication cell human body. also included list vital essential medicines, which confirms importance for Russian healthcare this drug fight co...

A procedure for simultaneous identification and quantification of canrenone and its biotransformed product 11-α-hydroxy-canrenone by high-performance liquid chromatography with ultraviolet detector (HPLC-UVD) and mass spectrometry (LC-MS) methods was proposed. The optimal determination variables on the HPLC-UVD or LC-MS coupled with a ZORBAX Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μm) were s...

We describe a simple, sensitive, and specific high-performance liquid-chromatographic method with ultraviolet detection (256 nm) for the simultaneous analysis of nicotine and cotinine in urine of passive smokers. The analytes are extracted and purified from the complex and impure matrix in two stages; first, by liquid-liquid extraction and followed by solid-phase extraction (C2 column). We used...

BACKGROUND AND THE PURPOSE OF THE STUDY Melatonin has recently been found in several plant tissues. Some reports show that the majority of the herbs containing the high level of melatonin have been used traditionally to treat neurological disorders or diseases associated with the generation of free radicals. Current study was undertaken to screen some medicinal plant species with historical evi...

Hydrochlorothiazide (HCT) is a thiazide diuretic which reduces the resorption of electrolytes and consequently of water. Enalapril maleate (EM) is a derivative of 2 amino acids, L-alanine and L-proline, and is an antihypertensive and a vasodilator in congestive heart failure. The 2 drugs are used in association in the treatment of hypertension . Several methods are available in the literature f...

AIM Quantitative estimation of lercanidipine HCl in bulk material as well as from nanosuspension formulations via a developed reverse phase HPLC method. MATERIALS AND METHODS Optimized chromatographic condition was used to achieve separation on a Kromasil (100-5c18 250 × 4.6 mm) column using Shimadzu HPLC system. The mobile phase consisted of a mixture of acetate buffer (20 mM pH 4.5) and ace...

Selegiline is best known as an irreversible inhibitor of monoamine oxidase (MAO), an intracellular enzyme associated with the outer membrane of mitochondria. The purpose of this study is to establish a reliable and quick method for the assignment of selegiline in human plasma by high performance liquid chromatography with ultraviolet detection. A rapid and sensitive high performance liquid chro...

Valnemulin, successfully developed by Sandoz in 1984, is a new generation derivative of pleuromutilin related to tiamulin. Valnemulin has low water-solubility, a short half-life period, low bioavailability, and instability. The application of valnemulin was restricted. Therefore, finding a more moderate delivery system is necessary to improve the shortcomings of valnemulin. The purpose of the s...

Using high-performance liquid chromatography (HPLC) and 2,4-dinitrophenylhydrazine (2,4-DNPH) as a derivatizing reagent, an analytical method was developed for the quantitative determination of acetone in human blood. The determination was carried out at 365 nm using an ultraviolet-visible (UV-Vis) diode array detector (DAD). For acetone as its 2,4-dinitrophenylhydrazone derivative, a good sepa...