Aim of the present investigation was to assess the in vitro efficacy of different plant extracts againsts fungi isolated from bovine mastitis cases. Twenty-six species of yeast, yeast like and moulds were isolated from 200 mastitic milk samples. The isolates were identified based on the staining, cultural and biochemical characteristics. Aqueous extracts of cinnamon leaf, lemon grass, clove, an...

In order to explore the biochemical scope of ochratoxin A-producing penicillia, we screened 48 Penicillium verrucosum isolates for the production of secondary metabolites. Fungal metabolites were analyzed by high-pressure liquid or gas chromatography coupled to diode array detection or mass spectrometry. The following metabolites were identified: ochratoxins A and B, citrinin, verrucolones, ver...

The performance of the RapID Yeast Plus System (Innovative Diagnostic Systems, Norcross, Ga.), a 4-h micropanel using single-substrate enzymatic test reactions, was compared with that of the API 20C AUX Clinical Yeast System (bioMerieux Vitek, Hazelwood, Mo.), a 48- to 72-h carbohydrate assimilation panel. Two hundred twenty-five yeasts, yeast-like fungi, and algae, comprising 28 species and in...

Mandarin fruits are one of the most popular among Citrus genus. They consumed because their nutritional and health benefits, as well pleasant taste smell. This paper describes identification characterization Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucros...

We have conducted a longitudinal study over a 3-year period to address the point prevalence, microbiological characteristics and antifungal susceptibility patterns of yeast isolates colonizing or infecting the oral cavities of 111 HIV-infected (51 adults, 60 children) and 201 non HIV-infected (109 adults, 92 children) Mexican persons. Regarding the epidemiology of oral candidiasis, Candida albi...

With recent advancements of both biological and computer technologies, large-scale experiments are on the point of being undertaken. However, it is unlikely that the experiments will be sufficient to replace traditional studies where a single molecule is examined. For instance, several methods attempt to reconstruct a gene regulatory network from expression measurement data do not explore the p...

Data of protein-protein interactions provide valuable insight into the molecular networks underlying a living cell. However, their accuracy is often questioned, calling for a rigorous assessment of their reliability. The computation offered here provides an intelligible mean to assess directly the rate of true positives in a data set of experimentally determined interacting protein pairs. We sh...

Protein-protein interactions (PPIs) play crucial roles in protein function for a variety of biological processes. Data from large-scale PPI screening has contributed to understanding the function of a large number of predicted genes from fully sequenced genomes. Here, we report the systematic identification of protein interactions for the unicellular cyanobacterium Synechocystis sp. strain PCC6...

Mutations A49P and Delta47-53 at the T loop of the Escherichia coli GlnB (PII) protein impair regulatory interactions with the two-component sensor regulator NtrB (P. Jiang, P. Zucker, M. R. Atkinson, E. S. Kamberov, W. Tirasophon, P. Chandran, B. R. Schepke, and A. J. Ninfa, J. Bacteriol. 179: 4342-4353, 1997). We show here that these mutations also impair interactions between PII and NtrB in ...

the virulence of four iranian isolates and one exotic isolate of beauveria bassiana on the fifth instar nymphs and also five iranian isolates and one exotic isolate of it on the adults of eurygaster integriceps puton was studied using dipping and topical micro-application techniques, respectively. nymphs were highly susceptible to the isolates. comparison of lc50 values showed no significant di...