Cathepsin B is a lysosomal cysteine protease, contributing to vital cellular homeostatic processes including protein turnover, macroautophagy of damaged organelles, antigen presentation, and in the extracellular space, it takes part tissue remodeling, prohormone processing, activation. However, aberrant overexpression cathepsin its enzymatic activity associated with different pathological condi...

Abstract One of the key challenges in development CAR-T therapy is absence target antigens that are tumor-specific and have homogenous expression. To safely glioblastoma-associated (GAAs) tumor without attacking normal tissue expressing same GAAs outside brain, we adapted a novel synthetic Notch (synNotch) receptor system established “prime kill” sequential two-receptor CAR circuit. We used gli...

in this study one lymph node from cow with viral lymphosarcoma and one lymph node from healthy cow were analyzed by three different methods for protein extraction, include simple homogenizing in pbs, sonication and phenol extraction. tumor antigen detection relies on immunoprecipitation followed by sds-page. in this experiment streptococcus pyogenes was used for purification of immune complexes...