purification of parasitic antigens is a major activity in immunoparasitology because of its application in immunodiagnosis, vaccination analysis of immunopathology, preparation of monocolomal antibody and finding out the cross reactive antigens versus specific antigens of a parasite. in this survey f2 ultrafiltration excretory secretory antigens of ascaris lumbricoides and toxocara cati were se...

background: it is relevant to highlight that there is not a precise and perfect report on either 95 percentile value (upper limit of normal range) or on appropriate reference intervals for serum psa in iranian population. objective: to determine age-specific reference ranges for serum prostate-specific antigen (psa) concentration and psa density (psad) and prostate volumes in a population of he...

objectives: ctxb (cholera toxin b subunit) contributes to a vaccine's efficacy by stimulating production of the anti-ctxb antibody. various attempts have been made to increase production of this antibody. chitosan is a mucoadhesive polysaccharide that has tremendous potential for oral vaccine delivery in terms of its exclusive features that include biocompatibility, biodegradability, high charg...

clinical asthma and airway responsiveness appear to be less severe when diabetes is superimposed. the aim of the present study was to determine the possible role of nitric oxide (no) in the airway reactivity under diabetic and diabetic-allergic conditions. twenty-five male guinea-pigs were divided into five groups of five each as follows: diabetic, antigen sensitized, diabetic- antigen sensitiz...

Introduction: The major histocompatibility complex (MHC) is a group of cell surface proteins that are essential for recognizing foreign molecules in human and other mammals. The physiologic function of MHC molecules is the presentation of peptides to T cells. In this study, we evaluated the purification of a class II MHC molecule (HLA-DR) from a human Burkitt′s lymphoma cell line; Daudi...

Processed antigen reacts selectively with lymphocytes bearing .appropriate surface immunoglobulin molecules resulting in the clonal proliferation of these cells, produc­ing circulating immunoglobulin (humoral immunity) or non-circulating immunoglobulins (cellular immunity).  High levels of circulating immunoglobulins (lgM & lgG) occur in protozoa and helminths infections. Reaginic antibodies a...

the holstein bulls (n=50) were genotyped for bovine lymphocyte antigen (bola-drb3.2) alleles by polymerase chain reaction and restriction fragment length polymorphism (pcr-rflp). genomic dna was extracted from bull semen using phenol-chloroform method. a two-step pcr was conducted in order to amplify a 284 base-pair fragment of the target gene. amplicons were digested by rsai, haeiii and bstyi ...

hydatid cyst fluid has been used as a source of antigen for the serodiagnosis of echinococcus granulosus infection. due to cross-reactions with antigens shared by other helminthes, the specific antigen from hydatid cyst fluid was purified by many workers. we used a relatively simple technique for purification of specific antigen from sheep hydatid cyst fluid. isopycnic ultracentrifugation with ...