The solution structure of bovine pancreatic trypsin inhibitor (BPTI) at a pressure of 2 kbar is presented. The structure was calculated as a change from an energy-minimized low-pressure structure, using (1)H chemical shifts as restraints. The structure has changed by 0.24 A RMS, and has almost unchanged volume. The largest changes as a result of pressure are in the loop 10-16, which contains th...

Differential relaxation of multiple quantum coherences is a signature for chemical exchange processes in proteins. Previous analyses of experimental data have used theoretical descriptions applicable only in the limit of fast exchange. Theoretical expressions for differential relaxation rate constants that are accurate outside fast exchange are presented for two-spin-system subject to two-site ...

The amino acid sequences of garden bean (Phaseolus vulgaris) trypsin inhibitor II and a related molecular species II' have been examined. The entire sequence of II' has been determined with the exception of five internal residues. The garden bean inhibitors are highly homologous to the Bowman-Birk soybean inhibitor and lima bean trypsin inhibitor IV. The trypsin-reactive site has been located i...

The wide-spread distribution of trypsin inhibitors throughout the vegetable and animal kingdoms (1) makes elucidation of their physiological function of great interest. From this point of view, resistance to peptic digestion becomes an important property, since only an inhibitor resistant to pepsin is likely to produce any physiological effect when ingested by a normal animal. The soy bean inhi...

The existence of a trypsin-like enzyme (TLE) in acrosomes ofepididymal spermatozoa was confirmed and was further demonstrated to be present in acrosomes of ejaculated and capacitated spermatozoa. TLE rapidly removes the zona pellucida of the ovum. Extracts of acrosomes of ejaculated spermatozoa contain an inhibitor that is separated from the TLE by purification of the TLE. The inhibitor of TLE ...

The Mustard Trypsin Inhibitor (MSI) family is a small family of plant protease inhibitors so far only found in Brassicaceae. Using a phage display selection, MTI-2 (Mustard Trypsin Inhibitor 2) mutants were detected and analysed for their biochemical characteristics. Retromutants of the selected MTI-2 proteins were constructed and expressed in the Pichia pastoris system. The recombinant protein...

il study of enzyme inhibitors designed to obtain information on the possible physiological significance of blood antiprotease has produced a number of simple fundamental reactions which have hitherto not been reported. It is the purpose of this paper to record these reactions for the possible benefit of others who may wish to investigate them further. Materials-The t,rypsin, chymotrypsin, and t...

Ham and Sandstedt (2) and, subsequently, others have shown that there is a trypsin inhibitor in soy beans and other legumes which inhibits the growth of certain animals. At first it was believed that the components of beans which alter the nutritive value of proteins exert growth inhibition by their ability to inhibit proteolysis in the gastrointestinal tract. Recently Klose and associates (3) ...

Serum protease inhibitor activity was assessed from the ability of the serum to inhibit casein hydrolysis by ct-chymotrypsin (EC 3.4.4.5). Unmodified AutoAnalyzer equipment was used in conjunction with a fluorimetric method for dialyzable tyrosyl peptides. Serum, diluted with tris(hydroxymethyl) aminomethane buffer, was added to a chymotrypsin solution to inhibit its activity. For 72 sera, the ...