Purpose: The aim of this study was to determine the effects Echinacoside on cell proliferation, invasion and mRNA expression changes invasion-related genes in PC3 androgen-independent prostate cancer cells.
 Material Methods: effect proliferation cells determined by XTT method. Anti-invasive efficacy achieved using transwell chamber. Total RNA isolation performed Trizol cDNA subsequently s...

Submit Manuscript | http://medcraveonline.com extraction of RNA in particular mRNA which is protein coding [1]. The RNA in total need to be protein free, intact, free of inhibitor enzymes which could interfere with the downstream segments of transcripts [2,3]. Hence, the quality, purity and integrity of extracted RNAs beside the quantity are the values which are under improvement of developing ...

Allergic airway inflammation and asthma have emerged as major public health problem in the Midwest, United States of America. Targeting Toll-like receptor (TLR) signaling and Calcium-activated Chloride (ClCa)/Potassium (KCa) Channels in unraveling the cellular/molecular mechanisms underlying genetic susceptibility to inflammatory diseases in specific human patient population subset(s) is emergi...

Since RNA extraction is a crucial step in many molecular techniques, the protocols for sample collection and RNA purification need to be adapted to optimize their performance when samples are collected from animals at commercial facilities. Here we provide an RNA purification protocol for animal tissues collected from slaughterhouses. This protocol, modified from other techniques, uses TRI...

Background: N-heptyl-D-galactonamide (GalC7) is a small synthetic carbohydrate derivative that forms biocompatible supramolecular hydrogel. In this study, the objective was to analyze more in-depth how neural cells differentiate in contact with GalC7. Method: Direct (ex vivo) of fresh hippocampus and culture (In vitro) primary were investigated. vitro, investigation performed under three condit...

We developed an alternative method to extract DNA and RNA from clotted blood for genomic and molecular investigations. A combination of the TRIzol method and the QIAamp spin column were used to extract RNA from frozen clotted blood. Clotted blood was sonicated and then the QIAamp DNA Blood Mini Kit was used for DNA extraction. Extracted DNA and RNA were adequate for gene expression analysi...

We attempted to improve a protocol to allow for the consistent extraction of total RNA from prokaryotic cells. Total RNA was isolated from Escherichia coli B23 and yeast cells using Trizol. In the comparison of two different protocols it was found that performing the phase separation independent from the bead bashing results in increased yield and purity of the RNA. We also attempted to improve...

In this study, four protein extraction protocols from Mytilus galloprovincialis were evaluated with the aim to identify the most practical, efficient and reproducible method. Four extraction protocols frequently used for mussels and organic matrices were selected and compared. The methods were based on the use of: i) TRIzol reagent; ii) Lysis buffer; iii) phenylmethanesulfonyl fluoride; iv) tri...

Long non-coding RNAs (lncRNAs) have been demonstrated to be significant in numerous biological processes. Hypertension is a form of cardiovascular disease with at least one billion cases worldwide. The present study sought to compare the differential expression profiles of lncRNAs in the renal cortex of spontaneously hypertensive rats (SHRs) and normotensive Wistar‑Kyoto (WKY) rats. The ipsilat...